Increased LEF1 expression and decreased Notch2 expression are strong predictors of poor outcomes in colorectal cancer patients.
Increased LEF1 expression and decreased Notch2 expression are strong predictors of poor outcomes in colorectal cancer patients.
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LEF1 表达增加和 Notch2 表达减少是结直肠癌患者预后不良的有力预测因素。
DOI：
10.1155/2013/983981
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Nan KJ
中科院分区：
文献类型：
--
作者：
Wang WJ;Yao Y;Jiang LL;Hu TH;Ma JQ;Ruan ZP;Tian T;Guo H;Wang SH;Nan KJ
背景/目标。我们的目的是研究淋巴细胞增强因子1 （LEF1）和Notch2在结直肠癌（CRC）中的表达及其与临床病理变量和结直肠癌患者预后的关系。方法。采用免疫组织化学、定量实时聚合酶链反应（qRT-PCR）和Western blot分析184例结直肠癌患者中LEF1和Notch2的表达。结果。我们观察到LEF1表达与Notch2表达呈显著负相关（P < 0.001）。与匹配的瘤旁正常组织相比，肿瘤组织中LEF1 mRNA和蛋白表达升高，Notch2 mRNA和蛋白表达降低（P < 0.001）。LEF1蛋白表达的增加与淋巴结转移、远处转移、晚期TNM（肿瘤-淋巴结-转移）分期和较短的总生存期显著相关。Notch2蛋白表达降低与肿瘤低分化、淋巴结转移、远处转移、TNM晚期和患者总生存期缩短相关。多因素Cox回归分析发现，LEF1蛋白表达（P < 0.001）、Notch2蛋白表达（P < 0.001）、TNM分期（P < 0.001）以及LEF1蛋白共表达升高和Notch2蛋白共表达降低的联合表达（P < 0.001）是CRC的独立预后指标。结论。我们的研究结果表明，LEF1共表达升高和Notch2共表达降低是CRC患者总生存率较差的一个危险因素。
Background/Objective. We aimed to examine the expression of lymphoid enhancer factor 1 (LEF1) and Notch2 in colorectal cancer (CRC) and their association with clinicopathologic variables and CRC patients' prognosis. Methods. Immunohistochemistry, quantitative real-time polymerase chain reaction (qRT-PCR), and Western blot analysis were performed to assess the expression of LEF1 and Notch2 in 184 patients with CRC. Results. We observed a strong negative correlation between LEF1 expression and Notch2 expression (P < 0.001). Both LEF1 mRNA and protein expression increased while the Notch2 mRNA and protein expression decreased in tumor specimens compared with the matched paratumorous normal tissue (P < 0.001). An increase in LEF1 protein expression was significantly associated with lymph node metastases, distant metastasis, advanced TNM (tumor-node-metastasis) stage, and shorter overall survival. A decrease in Notch2 protein expression was associated with poorly differentiated tumors, lymph node metastases, distant metastasis, advanced TNM stage, and shorter overall survival of patients. In the multivariate Cox regression analysis, the LEF1 protein expression (P < 0.001), Notch2 protein expression (P < 0.001), TNM stage (P < 0.001), and the combination of increased LEF1 protein coexpression and decreased Notch2 protein coexpression (P < 0.001) were found to be independent prognostic indicators in CRC. Conclusion. Our results suggest that increased LEF1 coexpression and decreased Notch2 coexpression represent a risk factor for poor overall survival of CRC patients.
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影响因子：
4.6
作者：
Andreu, P;Colnot, S;Romagnolo, B
通讯作者：
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