Maturation of McjA precursor peptide into active microcin MccJ25
Maturation of McjA precursor peptide into active microcin MccJ25
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DOI：
10.1039/b708478a
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发表时间：
2007-01-01
影响因子：
3.2
通讯作者：
Campopiano, Dominic J.
中科院分区：
文献类型：
--
作者：
Clarke, David J.;Campopiano, Dominic J.
Microcin J25是由某些肠杆菌菌株产生的核糖体合成的21个残基的抗菌肽，其采用非凡的“螺纹套索”结构。迄今为止，这种肽的生物合成知之甚少。在这里，我们首次报道了微球蛋白前体肽在体外成熟为活性微球蛋白J25。此外，我们表明该前体肽的翻译后修饰所需的酶与细菌内膜相关。
Microcin J25 is a ribosomally synthesised 21- residue antimicrobial peptide produced by certain strains of enterobacteria, that adopts an extraordinary 'threaded lasso' structure. To date, the biosynthesis of this peptide is little understood. Here we report the in vitro maturation of the microcin precursor peptide into active microcin J25 for the first time. Furthermore, we show that the enzymes required for the posttranslational modification of this precursor peptide are associated with the bacterial inner membrane.