Imbalance of Gut Streptococcus, Clostridium, and Akkermansia Determines the Natural Course of Atopic Dermatitis in Infant
Imbalance of Gut Streptococcus, Clostridium, and Akkermansia Determines the Natural Course of Atopic Dermatitis in Infant
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DOI：
10.4168/aair.2020.12.2.322
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发表时间：
2020-03-01
影响因子：
4.4
通讯作者：
Hong, Soo-Jong
中科院分区：
文献类型：
--
作者：
Park, Yoon Mee;Lee, So-Yeon;Hong, Soo-Jong
目的：肠道微生物区系在特应性皮炎(AD)自然病程中的作用尚不完全清楚。为探讨6个月大婴儿肠道微生物区系和短链脂肪酸(SCFAs)的组成和功能是否会影响儿童早期AD的自然病程。方法：应用焦磷酸测序技术对84例健康对照、22例暂时性AD和26例持续性AD患儿的粪便样本进行焦磷酸测序分析。通过全基因组测序分析肠道微生物组的功能图谱。结果：一过性AD患儿均表现为低水平的链球菌和高含量的阿克曼杆菌，持续性AD患儿存在低水平的梭状芽胞杆菌、高水平的阿克曼杆菌和高度的链球菌。链球菌的相对丰度与AD(SCORAD)评分呈正相关，梭菌的相对丰度与SCORAD评分呈负相关。与健康对照组相比，持续性ALL组与氧化磷酸化相关的肠道微生物功能基因减少。暂时性ALL患儿的丁酸和戊酸水平低于健康和持续性AD患儿。结论：ALL患儿早期肠道菌群的组成、功能和代谢产物与ALL的自然病程有关。
Purpose: The roles of gut microbiota on the natural course of atopic dermatitis (AD) are not yet fully understood. We investigated whether the composition and function of gut microbiota and short-chain fatty acids (SCFAs) at 6 months of age could affect the natural course of AD tip to 24 months in early childhood.Methods: Fecal samples from 132 infants were analyzed using pyrosequencing, including 84 healthy controls, 22 transient AD and 26 persistent AD subjects from the Cohort for Childhood Origin of Asthma and Allergic Diseases (COCOA) birth cohort. The functional profile of the gut microbiome was analyzed by whole-metagenome sequencing. SCFAs were measured using gas chromatography-mass spectrometry.Results: Low levels of Streptococcus and high amounts of Akkermansia were evident in transient All cases, and low Clostridium, Akkermansia and high Streptococcus were found in children with persistent AD. The relative abundance of Streptococcus positively correlated with scoring of AD (SCORAD) score, whereas that of Clostridium negatively correlated with SCORAD score. The persistent All group showed decreased gut microbial functional genes related to oxidative phosphorylation compared with healthy controls. Butyrate and valerate levels were lower in transient All infants compared with healthy and persistent AD infants.Conclusions: Compositions, functions and metabolites of the early gut microbiome are related to natural courses of All in infants.