Molecular identification of Anopheles (Cellia) sundaicus from the Andaman and Nicobar islands of India
Molecular identification of Anopheles (Cellia) sundaicus from the Andaman and Nicobar islands of India
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DOI：
10.1016/j.actatropica.2005.07.028
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发表时间：
2006-01-01
期刊：
影响因子：
2.7
通讯作者：
Sharma, YD
中科院分区：
文献类型：
--
作者：
Alam, MT;Das, MK;Sharma, YD
孙代按蚊（Anopheles（Cellia）sundaicus（Rodenwaldt））是印度安达曼和尼科巴群岛的一种重要疟疾媒介，在那里它在淡水和半咸水中繁殖。为了确定An的分子身份。我们分析了来自四个地理上孤立的地区--特雷萨岛、南科里岛、卡尔尼科巴岛和卡查尔岛的样本。对28 SrRNA的内转录间隔区2（ITS 2）和结构域3（D3）进行PCR扩增和核苷酸序列分析。ITS 2区的一个。四个岛屿的孙代克斯与安氏种完全相同，但又有差异。sundaicus A.和越南南方古猿An. Sundaicus s.s.马来西亚。此外，淡水和微咸水形式的An。Sundaicus没有发现任何序列变异。类似地，D3序列在所有An中是相同的。四个岛屿的巽他鱼样本。D3序列的一个物种的Sundaicus复合体在这里首次报道，因此不能与该物种的其他区域分离物进行比较。因此，在安达曼和尼科巴群岛上可能只有一个Sundaicus复合体的成员存在，它在淡水和半咸水中繁殖，并且不同于An。sundaicus A和Malaysian An.孙代克斯有限公司从病媒控制战略的角度来看，在这些岛屿上确定一个新的Sundaicus复合体的兄弟物种是有意义的。(C)2005 Elsevier B. V.保留所有权利。
Anopheles (Cellia) sundaicus (Rodenwaldt) is an important malaria vector in the Andaman and Nicobar islands of India where it breeds in freshwater as well as in brackish water. To establish the molecular identity of An. sundaicus on these islands we analyzed samples from four geographically isolated areas-Teressa, Nancowry, Car Nicobar and Katchal islands. PCR-amplification and nucleotide sequence analysis were performed for internal transcribed spacer 2 (ITS2) and domain-3 (D3) of 28S rRNA. The ITS2 region of An. sundaicus from all four islands was identical but different from An. sundaicus A of Vietnam and An. sundaicus s.s of Malaysia. Furthermore, freshwater and brackish water forms of An. sundaicus did not reveal any sequence variation. Similarly, the D3 sequences were identical among all An. sundaicus samples from the four islands. D3 sequences for a species of the Sundaicus Complex are reported here for the first time and thus could not be compared with other regional isolates of this species. In conclusion, probably only one member of the Sundaicus Complex exists on the Andaman and Nicobar islands, which breeds in freshwater as well as in brackish water and is different from the An. sundaicus A and Malaysian An. sundaicus s.s. The identification of a new sibling species of the Sundaicus Complex in these islands is significant from the viewpoint of vector control strategies. (C) 2005 Elsevier B.V. All rights reserved.