Highly adherent small-colony variants of Pseudomonas aeruginosa in cystic fibrosis lung infection
Highly adherent small-colony variants of Pseudomonas aeruginosa in cystic fibrosis lung infection
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DOI：
10.1099/jmm.0.05069-0
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发表时间：
2003-04-01
影响因子：
3
通讯作者：
Steinmetz, I
中科院分区：
文献类型：
--
作者：
Häussler, S;Ziegler, I;Steinmetz, I
铜绿假单胞菌是一种机会性人类病原体和普遍存在的环境细菌，能够形成专门的细菌群落，称为生物膜。这项研究的结果表明，囊性纤维化（CF）肺的独特环境似乎选择了一个自我聚集和过度增生的铜绿假单胞菌小集落变体（SCV）亚群。与体外从 SCV 群体中分离的克隆野生型和快速生长的回复体相比，这些形态型在稳定生长条件下表现出更高的适应性。与过度纤毛的 SCV 一致，它们表现出增加的抽搐运动和生物膜形成能力。此外，SCV 与肺细胞系 A549 牢固附着。 CIF 肺内这些高度粘附的 SCV 的出现可能在铜绿假单胞菌肺部感染的发病机制中发挥关键作用，其中生物膜生长模式被认为是造成持续感染的原因。
Pseudomonas aeruginosa, an opportunistic human pathogen and ubiquitous environmental bacterium, is capable of forming specialized bacterial communities, referred to as biofilm. The results of this study demonstrate that the unique environment of the cystic fibrosis (CF) lung seems to select for a subgroup of autoaggregative and hyperpiliated P. aeruginosa small-colony variants (SCVs). These morphotypes showed increased fitness under stationary growth conditions in comparison with clonal wild-types and fast-growing revertants isolated from the SCV population in vitro. In accordance with the SCVs being hyperpiliated, they exhibited increased twitching motility and capacity for biofilm formation. In addition, the SCVs attached strongly to the pneumocytic cell line A549. The emergence of these highly adherent SCVs within the CIF lung might play a key role in the pathogenesis of P. aeruginosa lung infection, where a biofilm mode of growth is thought to be responsible for persistent infection.