AN APPLICATION OF SCANNED FOCUSED ION-BEAM MILLING TO STUDIES ON THE INTERNAL MORPHOLOGY OF SMALL ARTHROPODS
AN APPLICATION OF SCANNED FOCUSED ION-BEAM MILLING TO STUDIES ON THE INTERNAL MORPHOLOGY OF SMALL ARTHROPODS
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DOI：
10.1111/j.1365-2818.1993.tb03396.x
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发表时间：
1993-10-01
期刊：
影响因子：
--
通讯作者：
PUGH, PJA
中科院分区：
文献类型：
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作者：
YOUNG, RJ;DINGLE, T;PUGH, PJA
直径约 50 nm 的扫描聚焦离子束首次用于制备生物材料。通过离子蚀刻去除表面的小限定区域，以便用扫描电子显微镜检查下面的结构。对 Halarachne 属螨虫（Halarachnidae：Mesostigmata）的两个解剖特征进行了不同的研磨程序。首先，在周围板的表面铣出方孔，以揭示下面的呼吸周围沟的结构。在第二个中，进行横向切割：穿过构成感觉的感觉器的轴。跗骨 I 上的感觉哈勒氏器官。后一个程序揭示了感觉器核心和壁内的细节。介绍了样品制备和研磨程序的详细信息，以及结果的适用性和解释。
For the first time a scanned focused ion beam of approximately 50 nm diameter has been used to prepare biological material. Small defined areas of the surface were removed by ion etching to allow examination of the underlying structures with a scanning electron microscope. Different milling procedures were carried out on two anatomical features in mites of the genus Halarachne (Halarachnidae: Mesostigmata). In the first, square holes were milled into the surface of the peritrematal plate to reveal the structure of the underlying respiratory peritrematal groove. In the second, transverse cuts were made: across the shafts of the sensory sensilli which make up the.; sensory Haller's organ on tarsus I. This latter procedure revealed detail both within the core and walls of sensilli. Details of specimen preparation and milling procedures, as well as suitability and interpretation of results, are presented.