Immature neurons in the primate amygdala: changes with early development and disrupted early environment.
Immature neurons in the primate amygdala: changes with early development and disrupted early environment.
复制标题
灵长类杏仁核中的未成熟神经元：随着早期发育和早期环境的破坏而变化。
DOI：
10.1101/2023.02.10.528076
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Fudge,JulieL
中科院分区：
文献类型：
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作者：
McHale-Matthews,AlexandraC;DeCampo,DanielleM;Love,Tanzy;Cameron,JudyL;Fudge,JulieL
在人类和非人类灵长类动物中，杏仁核旁层核（PL）含有未成熟的神经元。为了探索 PL 在发育过程中细胞生长的潜力，我们比较了 (1) 婴儿和青少年猕猴（对照，母性喂养）以及 (2) 在生命第一个月经历与母亲分离的婴儿猕猴与对照母性喂养婴儿的 PL 神经元。在母系饲养的动物中，与婴儿 PL 相比，青少年 PL 具有更少的未成熟神经元、更多的成熟神经元和更大的未成熟体体积。与婴儿 PL 相比，青少年 PL 中的总神经元（未成熟的和成熟的）也更少，这表明一些神经元在青春期时移出了 PL。母亲分离并没有改变婴儿 PL 中未成熟或成熟神经元的平均计数。然而，在所有幼年动物中，未成熟神经元体体积与成熟神经元计数密切相关。 TBR1 mRNA 是谷氨酸能神经元成熟所需的转录本，在母体分离的婴儿 PL 中显着减少（DeCampo 等，2017），并且与婴儿 PL 中的成熟神经元计数呈正相关。我们得出的结论是，未成熟的神经元在青春期逐渐成熟，而母体分离的压力可能会改变这一轨迹，正如动物中 TBR1 mRNA 与成熟神经元数量之间的相关性所揭示的那样。
In human and nonhuman primates, the amygdala paralaminar nucleus (PL) contains immature neurons. To explore the PL’s potential for cellular growth during development, we compared PL neurons in (1) infant and adolescent macaques (control, maternally-reared), and in (2) infant macaques that experienced separation from their mother in the first month of life compared to control maternally-reared infants. In maternally-reared animals, the adolescent PL had fewer immature neurons, more mature neurons, and larger immature soma volumes compared to infant PL. There were also fewer total neurons (immature plus mature) in adolescent versus infant PL, suggesting that some neurons move out of the PL by adolescence. Maternal separation did not change mean immature or mature neuron counts in infant PL. However, across all infant animals, immature neuron soma volume was strongly correlated with mature neuron counts. TBR1 mRNA, a transcript required for glutamatergic neuron maturation, is significantly reduced in the maternally-separated infant PL (DeCampo et al., 2017), and was also positively correlated with mature neuron counts in infant PL. We conclude that immature neurons gradually mature by adolescence, and that the stress of maternal separation may shift this trajectory, as revealed by correlations between TBR1 mRNA and mature neuron numbers across animals.