Prevention of diabetic nephropathy by treatment with astaxanthin in diabetic db/db mice
Prevention of diabetic nephropathy by treatment with astaxanthin in diabetic db/db mice
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DOI：
10.1002/biof.5520200105
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发表时间：
2004-01-01
期刊：
影响因子：
6
通讯作者：
Yoshikawa, T
中科院分区：
文献类型：
--
作者：
Naito, Y;Uchiyama, K;Yoshikawa, T
氧化应激被认为是糖尿病引起肾病的重要机制。虾青素是藻类、鱼类和鸟类中的常见色素，是一种具有显着抗氧化活性潜力的类胡萝卜素。在这项研究中，我们研究了长期服用虾青素是否可以预防小鼠氧化应激诱导的糖尿病肾病的进展。我们使用雌性 db/db 小鼠（2 型糖尿病的啮齿动物模型）及其非糖尿病 db/m 同窝小鼠。将小鼠分为三组：非糖尿病 db/m、糖尿病 db/db 和用虾青素治疗的糖尿病 db/db。实验期间测量血糖水平、体重、尿白蛋白和尿8-羟基脱氧鸟苷(8-OHdG)。从治疗开始起，进行了 12 周的组织学和 8-OHdG 免疫组织化学研究。治疗12周后，与未治疗的db/db组相比，虾青素治疗组的血糖水平较低；然而，与 db/m 小鼠相比，两组小鼠的水平均显着升高。与未治疗的db/db组相比，虾青素治疗组中通过系膜面积/总肾小球面积比计算的相对系膜面积显着改善。治疗12周时尿白蛋白和8-OHdG的增加被虾青素的长期治疗显着抑制。未处理的 db/db 小鼠肾小球中的 8-OHdG 免疫反应细胞比虾青素处理的 db/db 小鼠的肾小球数量更多。在这项研究中，虾青素治疗可改善 2 型糖尿病啮齿动物模型中糖尿病肾病的进展和加速。结果表明，虾青素的抗氧化活性可以减轻肾脏的氧化应激，防止肾细胞损伤。总之，施用虾青素可能是预防糖尿病肾病的新方法。
Oxidative stress is implicated as an important mechanism by which diabetes causes nephropathy. Astaxanthin, which is found as a common pigment in algae, fish, and birds, is a carotenoid with significant potential for antioxidative activity. In this study, we examined whether chronic administration of astaxanthin could prevent the progression of diabetic nephropathy induced by oxidative stress in mice. We used female db/db mice, a rodent model of type 2 diabetes, and their non-diabetic db/m littermates. The mice were divided into three groups as follows: non-diabetic db/m, diabetic db/db, and diabetic db/db treated with astaxanthin. Blood glucose level, body weight, urinary albumin, and urinary 8-hydroxydeoxyguanosine (8-OHdG) were measured during the experiments. Histological and 8-OHdG immunohistochemical studies were performed for 12 weeks from the beginning of treatment. After 12 weeks of treatment, the astaxanthin-treated group showed a lower level of blood glucose compared with the non-treated db/db group; however, both groups had a significantly high level compared with the db/m mice. The relative mesangial area calculated by the mesangial area/total glomerular area ratio was significantly ameliorated in the astaxanthin-treated group compared with the non-treated db/db group. The increases in urinary albumin and 8-OHdG at 12 weeks of treatment were significantly inhibited by chronic treatment with astaxanthin. The 8-OHdG immunoreactive cells in glomeruli of non-treated db/db mice were more numerous than in the astaxanthin-treated db/db mice. In this study, treatment with astaxanthin ameliorated the progression and acceleration of diabetic nephropathy in the rodent model of type 2 diabetes. The results suggested that the antioxidative activity of astaxanthin reduced the oxidative stress on the kidneys and prevented renal cell damage. In conclusion, administration of astaxanthin might be a novel approach for the prevention of diabetes nephropathy.