Tailored Silica-Antibiotic Nanoparticles: Overcoming Bacterial Resistance with Low Cytotoxicity
Tailored Silica-Antibiotic Nanoparticles: Overcoming Bacterial Resistance with Low Cytotoxicity
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DOI：
10.1021/la4046435
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发表时间：
2014-07-01
期刊：
影响因子：
3.9
通讯作者：
Cardoso, Mateus Borba
中科院分区：
文献类型：
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作者：
Capeletti, Larissa Brentano;de Oliveira, Luciane Franca;Cardoso, Mateus Borba
新的和更具侵略性的抗生素耐药细菌以惊人的速度出现，对全球卫生保健系统构成了日益严峻的挑战。因此，需要开发新的抗微生物剂来克服常规抗生素的无效性并绕过与这些病理相关的治疗限制。在这项研究中，我们提出了一种合成方案，这是能够捕获四环素抗生素到二氧化硅纳米球。这些结构的杀菌功效针对对抗生素敏感和耐药的细菌进行测试。对于非耐药细菌，我们的复合材料具有与游离四环素相当的杀菌效率。另一方面，合成的复合物能够避免耐药细菌的细菌生长，而游离四环素没有显示出显著的杀菌效果。最后，我们研究了这些纳米颗粒对哺乳动物细胞的细胞毒性，以检查任何可能的中毒效应。发现这些纳米球不是细胞增殖诱导剂，并且当与没有纳米颗粒的对照相比时，仅观察到细胞复制速率的降低。
New and more aggressive antibiotic resistant bacteria arise at an alarming rate and represent an ever-growing challenge to global health care systems. Consequently, the development of new antimicrobial agents is required to overcome the inefficiency of conventional antibiotics and bypass treatment limitations related to these pathologies. In this study, we present a synthesis protocol, which was able to entrap tetracycline antibiotic into silica nanospheres. Bactericidal efficacy of these structures was tested against bacteria that were susceptible and resistant to antibiotics. For nonresistant bacteria, our composite had bactericidal efficiency comparable to that of free-tetracycline. On the other hand, the synthesized composites were able to avoid bacterial growth of resistant bacteria while free-tetracycline has shown no significant bactericidal effect. Finally, we have investigated the cytotoxicity of these nanoparticles against mammalian cells to check any possible poisoning effect. It was found that these nanospheres are not apoptosis-inducers and only a reduction on the cell replication rate was seen when compared to the control without nanoparticles.