Interleukin-1β selectively increases substance P release and augments the ascending phase of the peristaltic reflex
Interleukin-1β selectively increases substance P release and augments the ascending phase of the peristaltic reflex
复制标题
DOI：
10.1046/j.1350-1925.2003.00445.x
复制
发表时间：
2003-12-01
影响因子：
3.5
通讯作者：
Grider, JR
中科院分区：
文献类型：
--
作者：
Grider, JR
肌条暴露于白细胞介素（IL）-1 β刺激P物质（SP）表达，表明IL-1 β与肠道炎症期间SP表达增加之间存在联系。本研究检测了IL-1 β诱导的SP表达是否通过增强SP释放和SP介导的反射活性来反映。暴露神经支配的纵向结肠肌条IL-1 β 8小时增加SP的合成，和更大的SP释放兴奋性运动神经元响应氯化钾或电场刺激（EFS），增强纵向肌肉收缩响应EFS。IL-1 Ra和IL-1 β抗体阻断IL-1 β诱导的SP释放和肌肉收缩的增加。血管活性肠肽（VIP）和生长抑素释放均未增加。SP释放的增加反映在响应拉伸的增强的环形肌肉收缩中。VIP介导的环肌下行松弛不受影响。IL-1 Ra或IL-1 β抗体可阻断IL-1 β诱导的选择性上升性收缩。我们的结论是，在兴奋性运动神经元的IL-1 β诱导的SP表达的选择性增加反映了增强SP释放和纵向肌肉收缩响应EFS，并增强SP释放和环形肌肉收缩在上升阶段的蠕动反射。
Exposure of muscle strips to interleukin (IL)-1beta stimulates substance P (SP) expression, suggesting a link between IL-1beta and the increase in SP expression during intestinal inflammation. The present study examined whether the SP expression induced by IL-1beta is reflected by enhanced SP release and SP-mediated reflex activity. Exposure of innervated longitudinal colonic muscle strips to IL-1beta for 8 h increased SP synthesis in, and greater SP release from excitatory motor neurones in response to KCl or electrical field stimulation (EFS), and enhanced longitudinal muscle contraction in response to EFS. IL-1 Ra and IL-1beta antibody blocked IL-1beta-induced increase in SP release and muscle contraction. Neither vasoactive intestinal peptide (VIP) nor somatostatin release was increased. The increase in SP release was reflected in enhanced circular muscle contraction in response to stretch. VIP-mediated descending relaxation of circular muscle was not affected. The selective increase in ascending contraction induced by exposure to IL-1beta was blocked by IL-1 Ra or IL-1beta antibody. We conclude that the selective increase in SP expression induced by IL-1beta in excitatory motor neurones is reflected by enhanced SP release and longitudinal muscle contraction in response to EFS, and enhanced SP release and circular muscle contraction during the ascending phase of the peristaltic reflex.