YY1 DNA binding and PcG recruitment requires CtBP
YY1 DNA binding and PcG recruitment requires CtBP
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DOI：
10.1101/gad.1228204
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发表时间：
2004-11-01
影响因子：
10.5
通讯作者：
Atchison, ML
中科院分区：
文献类型：
--
作者：
Srinivasan, L;Atchison, ML
我们发现哺乳动物Polycomb组（PcG）蛋白YY 1可以结合果蝇胚胎中的Polycomb反应元件，并可以招募其他PcG蛋白到DNA中。PcG募集导致组蛋白H3的去乙酰化和甲基化。在CtBP突变背景下，不会发生PcG蛋白的募集和伴随的组蛋白修饰。令人惊讶的是，体内YY1 DNA结合也被消除。CtBP突变不会导致YY 1降解或从细胞核转运，这表明YY 1 DNA结合能力被掩盖的机制。这些结果揭示了CtBP在控制YY1 DNA结合和PcG蛋白向DNA的募集中的新作用。
We found that mammalian Polycomb group (PcG) protein YY1 can bind to Polycomb response elements in Drosophila embryos and can recruit other PcG proteins to DNA. PcG recruitment results in deacetylation and methylation of histone H3. In a CtBP mutant background, recruitment of PcG proteins and concomitant histone modifications do not occur. Surprisingly, YY1 DNA binding in vivo is also ablated. CtBP mutation does not result in YY1 degradation or transport from the nucleus, suggesting a mechanism whereby YY1 DNA binding ability is masked. These results reveal a new role for CtBP in controlling YY1 DNA binding and recruitment of PcG proteins to DNA.