Lipid phosphate phosphatase activity regulates dispersal and bilateral sorting of embryonic germ cells in Drosophila
Lipid phosphate phosphatase activity regulates dispersal and bilateral sorting of embryonic germ cells in Drosophila
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DOI：
10.1242/dev.046110
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发表时间：
2010-06-01
期刊：
影响因子：
4.6
通讯作者：
Lehmann, Ruth
中科院分区：
文献类型：
--
作者：
Renault, Andrew D.;Kunwar, Prabhat S.;Lehmann, Ruth
在果蝇中，生殖细胞的存活和迁移方向由两种脂质磷酸磷酸酶（LPP）控制，wunen（wun）和wunen-2（wun 2）。wun wun 2双突变体分析揭示了这两个基因（以下统称为wunens）在原始生殖细胞中冗余地起作用。我们发现，wunens介导的生殖细胞生殖细胞排斥，这种排斥是必要的生殖细胞分散和适当的transepithelial迁移开始时的迁移和平等排序的生殖细胞之间的两个胚胎性腺在其迁移。我们建议，这种分散功能优化成人生殖力，确保最大的生殖细胞占用两个性腺。此外，我们发现，在生殖细胞的生存需要wunens可以通过阻止生殖细胞迁移消除。我们认为Wunen的这种基本功能是维持活跃迁移的生殖细胞的细胞完整性所必需的。
In Drosophila, germ cell survival and directionality of migration are controlled by two lipid phosphate phosphatases (LPP), wunen (wun) and wunen-2 (wun2). wun wun2 double mutant analysis reveals that the two genes, hereafter collectively called wunens, act redundantly in primordial germ cells. We find that wunens mediate germ cell-germ cell repulsion and that this repulsion is necessary for germ cell dispersal and proper transepithelial migration at the onset of migration and for the equal sorting of the germ cells between the two embryonic gonads during their migration. We propose that this dispersal function optimizes adult fecundity by assuring maximal germ cell occupancy of both gonads. Furthermore, we find that the requirement for wunens in germ cell survival can be eliminated by blocking germ cell migration. We suggest that this essential function of Wunen is needed to maintain cell integrity in actively migrating germ cells.