Cyclosporine inhibition of azaserine-induced atypical acinar cell foci in rat pancreas.
Cyclosporine inhibition of azaserine-induced atypical acinar cell foci in rat pancreas.
复制标题
环孢素抑制重氮丝氨酸诱导的大鼠胰腺非典型腺泡细胞灶。
DOI：
10.1097/00006676-199007000-00003
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发表时间：
1990
期刊：
影响因子：
2.9
通讯作者：
Longnecker,DS
中科院分区：
文献类型：
--
作者：
Shinozuka,H;Roebuck,BD;Longnecker,DS
采用非典型腺泡细胞灶的短期定量分析，研究了免疫抑制剂环孢霉素（CsA）对阿扎色林诱导的大鼠胰腺癌发生的影响。对14日龄的雄性刘易斯大鼠单次腹膜内注射阿扎胞苷（30 mg/kg）。在25日龄时，处理的大鼠断乳，并分为两组，喂食基础饲料或相同的饲料含有0.011%CsA。注射生理盐水的大鼠也喂食CsA饮食。注射重氮丝氨酸4个月后处死大鼠，量化胰腺中的嗜酸性和嗜碱性病灶。注射生理盐水和维持CsA饮食的大鼠胰腺组织学无明显变化。注射阿扎胞苷后，饮食CsA显着减少嗜酸性和嗜碱性病灶的数量。结论：CsA可抑制阿扎丝氨酸处理大鼠胰腺中起始细胞的生长，形成病灶。该实验模型可用于分析这种免疫抑制剂在上皮细胞肿瘤的诱导和生长中的修饰作用。
The effects of cyclosporine (CsA), an immunosuppressive agent, on azaserine-induced pancreatic carcinogenesis in rats were investigated using the short-term assays of the quantitation of atypical acinar cell foci. Male Lewis rats at 14 days of age were given a single intraperitoneal injection of azaserine (30 mg/kg). At 25 days of age, the treated rats were weaned and divided into two groups fed either basal diet or the same diet containing 0.011% CsA. Saline-injected rats were also fed the CsA diet. Rats were killed 4 months after azaserine injection and acidophilic and basophilic foci in the pancreas were quantified. The pancreas of the rats given saline injection and maintained on the CsA diet showed no significnt histological alterations. The dietary CsA after the injection of azaserine markedly reduced the number of both acidophilic and basophilic foci. It is concluded that addition of CsA to the diet inhibits the growth of initiated cells to form foci in the pancreas of azaserinetreated rats. The experimental model is useful in analyzing the modifying role of this immunosuppresant in the induction and growth of epithelial cell tumors.