Absence of HIV-1 DNA in high-risk seronegative individuals using high-input polymerase chain reaction.
Absence of HIV-1 DNA in high-risk seronegative individuals using high-input polymerase chain reaction.
复制标题
使用高输入聚合酶链反应检测高危血清阴性个体中 HIV-1 DNA 的缺失。
DOI：
10.1097/00002030-199110000-00008
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Busch,MP
中科院分区：
文献类型：
--
作者：
Lee,TH;el-Amad,Z;Reis,M;Adams,M;Donegan,EA;O'Brien,TR;Moss,AR;Busch,MP
在抗体阴性的高危个体中频繁感染HIV-1（所谓的“沉默”感染）的证据仍然存在争议。为了评估这些不一致的结果是否可能是血清转换前间歇性检测罕见感染细胞（低病毒载量）的结果，我们开发了一种改良的聚合酶链反应（PCR）技术，该技术能够分析比标准PCR高10倍的细胞DNA量（2× 10 6而不是0.2× 10 6输入细胞）。这种技术允许一致的检测HIV-1前病毒在两个血清阳性的个人谁反复测试阴性的标准输入PCR。然而，结果为阴性时，高输入PCR应用于51个标本从39个选定的高风险血清阴性个体。这些结果表明，病毒载量的变化之前或在没有血清转换可能不能解释不一致的证据，关于沉默的HIV-1感染。
Evidence of frequent HIV-1 infections in antibody-negative, high-risk individuals (so-called ‘silent’infections) remains controversial. To evaluate whether these discrepant results may be the consequence of intermittent detection of rare infected cells (low viral load) preceding seroconversion, we developed a modification of the polymerase chain reaction (PCR) technique which enabled analysis of 10-fold greater amounts of cellular DNA per reaction than standard PCR (2× 10 6 rather than 0.2× 10 6 input cells). This technique allowed consistent detection of HIV-1 provirus in two seropositive individuals who had repeatedly tested negative by standard-input PCR. However, results were negative when high-input PCR was applied to 51 specimens from 39 selected high-risk seronegative individuals. These results suggest that variations in viral load preceding or in the absence of seroconversion probably do not explain discrepant evidence regarding silent HIV-1 infection.