Analysis of the expression of growth factor, interleukin-1, and lactoferrin genes and the distribution of inflammatory leukocytes in the preimplantation mouse oviduct.
Analysis of the expression of growth factor, interleukin-1, and lactoferrin genes and the distribution of inflammatory leukocytes in the preimplantation mouse oviduct.
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着床前小鼠输卵管生长因子、白介素-1、乳铁蛋白基因的表达及炎性白细胞分布分析。
DOI：
10.1095/biolreprod51.4.597
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发表时间：
1994
影响因子：
3.6
通讯作者：
Andrews,GK
中科院分区：
文献类型：
--
作者：
Dalton,T;Kover,K;Dey,SK;Andrews,GK
输卵管提供了卵子受精和随后的植入前小鼠胚胎发育的环境，但对输卵管产生可能影响这些植入前事件的生长因子或细胞因子的能力知之甚少。采用北方印迹分析和/或免疫组织化学方法分别检测生长因子肝素结合表皮样生长因子（HB-EGF）、转化生长因子（TGF）α、表皮生长因子（EGF）、胰岛素样生长因子-I（IGF-I）、TGF β 1、TGF β2、TGFβ3、雌激素调节乳铁蛋白（LF）的表达或细胞分布;以及在着床前期（第1-4天[第1天=阴道栓]）和卵巢切除术后7天小鼠输卵管中细胞因子白细胞介素（IL）-Iα和IL- 13的变化。结果表明，除了EGF，每个生长因子和LF基因表达在壶腹和峡部地区的输卵管在整个植入前期。在分泌性上皮细胞中观察到HB-EGF、TGFα、IGF-I、TGFβ1和TGFβ2以及LF的显著免疫染色。还观察到浆膜和/或平滑肌中TGFα、IGF-I和TGFβ1的免疫染色强度较低。而TGFβ2在平滑肌细胞中表达较强，TGFβ 3仅在平滑肌细胞中表达。这些mRNA的丰度在着床前时期是相对恒定的，卵巢切除术并没有降低这些mRNA的水平。与这些生长因子相反，在这些实验条件下，检查的细胞因子mRNA（IL-1 α和IL-1β）等于或低于检测限，并且在输卵管中未检测到炎性白细胞（LF-免疫阳性中性粒细胞、IL-1 β-免疫阳性单核细胞/巨噬细胞或过氧化物酶阳性嗜酸性粒细胞），但在第1天在相邻子宫基质中大量存在。这些研究表明，几种生长因子是由小鼠输卵管合成的，并表明卵巢类固醇激素在着床前输卵管中调节这些基因的表达中不起主要作用。此外，与第1天的子宫不同，输卵管未表现出对交配的炎症反应。
The oviduct provides the environment in which fertilization of the egg and subsequent development of the preimplantation mouse embryo occurs, but little is known about the oviduct's capacity to produce growth factors or cytokines that may influence these preimplantation events. Northern blot analysis and/or immunohistochemistry were employed to examine the expression or cellular distribution, respectively, of the growth factors heparin-binding epidermal-like growth factor (HB-EGF), transforming growth factor (TGF) α, epidermal growth factor (EGF), insulin-like growth factor-I (IGF-I), TGFPβ1TGFβ2, and TGFβ3; of estrogen- regulated lactoferrin (LF); and of the cytokines interleukin (IL)-Iα and IL- 13 in the mouse oviduct during the preimplantation period (Days 1–4 [Day 1 = vaginal plug]) and 7 days after ovariectomy. The results demonstrated that, except for EGF, each of the growth factors and the LF genes are expressed in the ampulla and isthmus regions of the oviduct throughout the preimplantation period. Prominent immunostaining in secretory epithelial cells was noted for HB-EGF, TGFα, IGF-I, TGFβ1, and TGFβ2, and LF. Less intense immunostaining in the serosa and/or smooth muscle was also noted for TGFα, IGF-I, and TGFβ1. In contrast, intense immunostaining in smooth muscle was noted for TGFβ2, and TGFβ3was detected exclusively in smooth muscle cells. The abundance of these mRNAs was relatively constant during the preimplantation period, and ovariectomy did not reduce the levels of these mRNAs. In contrast to these growth factors, the cytokine mRNAs examined (IL-lα and IL-1β) were at or below the limits of detection under these experimental conditions, and inflammatory leukocytes (LF-immunopositive neutrophils, IL1β-immunopositive monocytes/macrophages, or peroxidase-positive eosinophils) were not detected in the oviduct, but were abundant in the adjacent uterine stroma on Day 1. These studies show that several growth factors are synthesized by the mouse oviduct and suggest that ovarian steroids do not play a major role in modulating expression of these genes in the oviduct during the preimplantation period. Furthermore, unlike the uterus on Day 1, the oviduct does not exhibit an inflammatory response to mating.