Comparative analysis of gastric bacterial microbiota in Mongolian gerbils after long-term infection with Helicobacter pylori
Comparative analysis of gastric bacterial microbiota in Mongolian gerbils after long-term infection with Helicobacter pylori
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DOI：
10.1016/j.micpath.2012.03.008
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发表时间：
2012-07-01
影响因子：
3.8
通讯作者：
Kamiya, Shigeru
中科院分区：
文献类型：
--
作者：
Osaki, Takako;Matsuki, Takahiro;Kamiya, Shigeru
16 SrDNA引物真实的时间定量PCR可用于蒙古沙鼠胃内菌群的细菌组成分析。本研究的目的是确定长期感染幽门螺杆菌后胃微生物群的变化。接种H. pylori感染，5只沙鼠被确定为H. pylori阳性，6只沙鼠H.通过培养和真实的时间qt PCR方法检测幽门螺杆菌阴性。并与6只未感染H.幽门螺杆菌。来自Atopobium簇，双歧杆菌属，球状梭菌群、纤细梭菌亚群、肠球菌属。和乳杆菌属。在感染和未感染的沙鼠的胃粘液中检测到。与此相反，圆柱状真杆菌组和普雷沃氏菌属。仅在H.幽门阴性沙鼠结果表明，在培养过程中，C leptum亚群、C coccoides群和双歧杆菌的数量均显著增加。在H. pylori阴性的蒙古沙鼠的感染率显著低于未感染的沙鼠。结果表明，蒙古沙鼠胃内固有菌群的组成可能受到长期感染H。pylori，并且这些变化实际上可能抑制H.幽门感染(C)2012爱思唯尔有限公司保留所有权利。
Quantitative (qt) real time PCR using 16SrDNA primers is useful for determination of the bacterial composition of the gastric microbiota in Mongolian gerbils. The aim of this study was to determine the change in the gastric microbiota after long-term infection with Helicobacter pylori. One year after inoculation with H. pylori, five gerbils were determined as H. pylori-positive and 6 gerbils H. pylori-negative by culture and real time qt PCR methods. The gastric microbiota of each group of gerbils was also compared with that of 6 gerbils uninfected with H. pylori. DNA from the Atopobium cluster, Bifidobacterium spp., Clostridium coccoides group, Clostridium leptum subgroup, Enterococcus spp. and Lactobacillus spp. were detected in the gastric mucus of both infected and uninfected gerbils. In contrast, Eubacterium cylindroides group and Prevotella spp. were detected only in H. pylori-negative gerbils. The numbers of C leptum subgroup, C coccoides group and Bifidobacterium spp. in gastric mucus of H. pylori-negative Mongolian gerbils were significantly lower than those in non-infected gerbils. The results obtained suggest that the composition of gastric indigenous microbiota in Mongolian gerbils may be disturbed by long-term infection with H. pylori, and that these changes may in fact inhibit H. pylori infection. (C) 2012 Elsevier Ltd. All rights reserved.