Insulin action during late pregnancy in the conscious dog.
Insulin action during late pregnancy in the conscious dog.
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妊娠晚期有意识的狗的胰岛素作用。
DOI：
10.1152/ajpendo.00143.2003
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发表时间：
2004
期刊：
影响因子：
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通讯作者：
Moore,MaryCourtney
中科院分区：
文献类型：
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作者：
Connolly,CynthiaC;Aglione,LisaN;Smith,MartaS;Lacy,DBrooks;Moore,MaryCourtney
我们的目的是评估外周胰岛素抵抗的程度，以及肝脏胰岛素作用的变化是否在妊娠晚期（妊娠晚期）犬模型中明显。使用清醒、禁食18 h的妊娠（P;n= 6）和非妊娠（NP;n= 6）雌性犬进行3 h高胰岛素血症（5 mU·kg−1·min−1）正葡萄糖钳夹试验，在试验前17天植入导管进行门静脉内胰岛素输注和肝脏底物平衡评估。第3 h动脉血浆胰岛素分别从11 ± 2 μU/ml上升到192 ± 24 μ U/ml和4 ± 2 μU/ml上升到178 ± 5 μU/ml。两组中胰高血糖素的下降幅度相同。NP组基础肝葡萄糖净输出量较低（1.9 ± 0.1 vs. 2.4 ± 0.2 mg·kg-1·min-1，P< 0.05）。高胰岛素血症完全抑制了两组的肝脏葡萄糖释放（NP和P分别为−0.4 ± 0.2和−0.1 ± 0.2 mg·kg−1·min− 1）。NP组需要更多的外源性葡萄糖才能维持正常血糖水平（15.2 ± 1.3 vs. 11.5 ± 1.1 mg·kg-1·min-1，P< 0.05）。非酯化脂肪酸在两组中下降相似。高胰岛素的净肝致纤维化氨基酸摄取在NP和P中没有差异。外周胰岛素作用在这种犬妊娠模型中明显受损，而肝葡萄糖产生完全被高循环胰岛素水平抑制。
Our aim was to assess the magnitude of peripheral insulin resistance and whether changes in hepatic insulin action were evident in a canine model of late (3rd trimester) pregnancy. A 3-h hyperinsulinemic (5 mU·kg−1·min−1) euglycemic clamp was conducted using conscious, 18-h-fasted pregnant (P;n= 6) and nonpregnant (NP;n= 6) female dogs in which catheters for intraportal insulin infusion and assessment of hepatic substrate balances were implanted ∼17 days before experimentation. Arterial plasma insulin rose from 11 ± 2 to 192 ± 24 and 4 ± 2 to 178 ± 5 μU/ml in the 3rd h in NP and P, respectively. Glucagon fell equivalently in both groups. Basal net hepatic glucose output was lower in NP (1.9 ± 0.1 vs. 2.4 ± 0.2 mg·kg−1·min−1,P< 0.05). Hyperinsulinemia completely suppressed hepatic glucose release in both groups (−0.4 ± 0.2 and −0.1 ± 0.2 mg·kg−1·min−1in NP and P, respectively). More exogenous glucose was required to maintain euglycemia in NP (15.2 ± 1.3 vs. 11.5 ± 1.1 mg·kg−1·min−1,P< 0.05). Nonesterified fatty acids fell similarly in both groups. Net hepatic gluconeogenic amino acid uptake with high insulin did not differ in NP and P. Peripheral insulin action is markedly impaired in this canine model of pregnancy, whereas hepatic glucose production is completely suppressed by high circulating insulin levels.