IN-SITU DETECTION OF PBS21 MESSENGER-RNA DURING SEXUAL DEVELOPMENT OF PLASMODIUM-BERGHEI
IN-SITU DETECTION OF PBS21 MESSENGER-RNA DURING SEXUAL DEVELOPMENT OF PLASMODIUM-BERGHEI
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DOI：
10.1016/0166-6851(94)90164-3
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发表时间：
1994-12-01
影响因子：
1.5
通讯作者：
SINDEN, RE
中科院分区：
文献类型：
--
作者：
THOMPSON, J;SINDEN, RE
用非放射性原位RNA杂交技术研究了伯氏疟原虫有性发育过程中核糖体RNA和编码寄生虫表面抗原Pbs21的mRNA的表达模式。一种与伯氏疟原虫核糖体RNA小亚基区域互补的RNA探针，在感染伯氏疟原虫的小鼠的血液涂片中与所有发育阶段的寄生虫杂交。相反，编码Pbs21的信使RNA只在脊椎动物宿主内致力于性发育的寄生虫中检测到，而且被证明以性别特异的方式表达，仅在雌配子细胞中表达。在性发育后期，Pbs21在雌配子和动子中的表达水平较高。我们以前已经证明，Pbs21蛋白只有在配子发生之后才能第一次检测到，配子发生发生在传播到昆虫载体之后。因此，这些结果表明，转录后机制调节Pbs21 mRNA在雌性配子体发生过程中积累的翻译。
The patterns of expression of ribosomal RNA and of mRNA encoding the parasite surface antigen Pbs21 have been investigated during the sexual stages of development of the malaria parasite, Plasmodium berghei, using the technique of non-radioactive in situ RNA hybridisation. An RNA probe complementary to a region of the small subunit of P. berghei ribosomal RNA hybridised to parasites at all stages of development in a smear of blood taken from mice infected with P. berghei. Messenger RNA encoding Pbs21, in contrast, was detected only within parasites committed to sexual development within the vertebrate host and, furthermore, was shown to be expressed in a sex-specific manner, exclusively within female gametocytes. At later stages of sexual development, Pbs21 mRNA was detected at high levels in female gametes and ookinetes. We have previously shown that Pbs21 protein is first detectable only after the initiation of gametogenesis which occurs following transmission to the insect vector. These results suggest, therefore, that post-transcriptional mechanisms operate to regulate the translation of Pbs21 mRNA as it accumulates during female gametocytogenesis.