A single layer nitrocellulose substrate for fabricating protein chips
A single layer nitrocellulose substrate for fabricating protein chips
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用于制造蛋白质芯片的单层硝化纤维基材
DOI：
10.1016/j.snb.2007.09.034
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发表时间：
2008
影响因子：
8.4
通讯作者：
Cheng
中科院分区：
文献类型：
--
作者：
L. Yin;Chih;Cheng
本文介绍了一种硅烷化化合物与硝化纤维素的形成，通过旋涂在载玻片上形成薄膜，可用作蛋白质芯片。该硝酸纤维素膜通过旋涂直接附着在载玻片上，同时通过疏水物理吸附和环氧化学键合来吸附蛋白质。使用这种旋涂薄膜形成技术，与原始FAST™载玻片相比，Cy 3和Cy 5通道背景都减少了90%。甲胎蛋白（AFP）抗原夹心实验结果表明，当AFP抗原浓度为10 ng/mL时，S/N为68.8，动态范围为30 ~ 3000 ng/mL。
This paper describes the formation of a silanization compound with nitrocellulose to form a thin film by spin coating on glass slide which can be used as a protein chip. This nitrocellulose film is adhered to the glass slide by spin coating directly and can immobilize protein by hydrophobic physical adsorption and epoxy chemical binding simultaneously. Using this spin coating thin film formation technique, both the Cy3 and Cy5 channel backgrounds are reduced by 90% when compared with the original FAST™ slide. The result of an alpha-1-foetoprotein (AFP) antigen sandwich experiment shows an S/N ratio of 68.8 in concentration of 10ng/mL of AFP antigen, and the dynamic range is between 30 and 3000ng/mL.