IL-33 is expressed in human osteoblasts, but has no direct effect on bone remodeling
IL-33 is expressed in human osteoblasts, but has no direct effect on bone remodeling
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DOI：
10.1016/j.cyto.2010.11.021
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发表时间：
2011-03-01
期刊：
影响因子：
3.8
通讯作者：
Magne, D.
中科院分区：
文献类型：
--
作者：
Saidi, S.;Bouri, F.;Magne, D.
本研究的目的是探讨最近发现的IL-1家族成员IL-33在骨重塑中的潜在作用。我们的研究结果表明，il - 33mrna在非炎性人骨的骨细胞中表达。此外，从三个健康供体获得的人骨髓基质细胞、成骨细胞和脂肪细胞中，tnf - α和IL-1 β增加了IL-33水平。用抑制剂GW-9662进行的实验表明，与IL-1 β相比，IL-33的表达不受PPAR γ的抑制，这可能解释了为什么IL-33而不是IL-1 β在脂肪细胞中表达。IL-33受体ST2L在人骨髓基质细胞、成骨细胞或cd14阳性单核细胞中不构成性表达，IL-33对这些细胞无影响。此外，骨髓基质细胞中ST2L mRNA虽然受到tnf - α和IL-1 β的诱导，但IL-33的作用与tnf - α和IL-1 β相同，因此IL-33的生物活性在该系统中可能是冗余的。与这一假设一致，MC3T3-E1成骨细胞样细胞组成性地表达ST2L mRNA， IL-33和tnf - α /IL-1 β类似地降低这些细胞中的骨钙素RNA水平。总之，我们的研究结果表明IL-33对正常骨重塑没有直接影响。(c) 2010 Elsevier Ltd.版权所有。
The aim of the present study was to investigate the potential role of the recently discovered IL-1 family member IL-33 in bone remodeling. Our results indicate that IL-33 mRNA is expressed in osteocytes in non-inflammatory human bone. Moreover, IL-33 levels are increased by TNF-alpha and IL-1 beta in human bone marrow stromal cells, osteoblasts and adipocytes obtained from three healthy donors. Experiments with the inhibitor GW-9662 suggested that expression of IL-33, in contrast to that of IL-1 beta, is not repressed by PPAR gamma likely explaining why IL-33, but not IL-1 beta, is expressed in adipocytes. The IL-33 receptor ST2L is not constitutively expressed in human bone marrow stromal cells, osteoblasts or CD14-positive monocytes, and IL-33 has no effect on these cells. In addition, although ST2L mRNA is induced by TNF-alpha and IL-1 beta in bone marrow stromal cells, IL-33 has the same effects as TNF-alpha and IL-1 beta, and, therefore, the biological activity of IL-33 may be redundant in this system. In agreement with this hypothesis, MC3T3-E1 osteoblast-like cells constitutively express ST2L mRNA, and IL-33 and TNF-alpha/IL-1 beta similarly decrease osteocalcin RNA levels in these cells. In conclusion, our results suggest that IL-33 has no direct effects on normal bone remodeling. (c) 2010 Elsevier Ltd. All rights reserved.