Pyramiding of bacterial blight resistance genes in rice: marker-assisted selection using RFLP and PCR
Pyramiding of bacterial blight resistance genes in rice: marker-assisted selection using RFLP and PCR
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DOI：
10.1007/s001220050565
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发表时间：
1997-08-01
影响因子：
5.4
通讯作者：
Khush, GS
中科院分区：
文献类型：
--
作者：
Huang, N;Angeles, ER;Khush, GS
利用DNA标记辅助选择技术对4个白叶枯病抗性基因Xa-4、Xa-5、Xa-13和Xa-21进行了聚合。开发了具有两个、三个和四个抗性基因的育种系，并测试了对细菌性枯萎病病原体（Xanthonzonas pv.））。与单基因品系相比，金字塔品系表现出更广的抗性谱和更高的抗性水平。为了加快基因扩增过程，并促进未来的标记辅助选择，我们开发了两个隐性基因，xa-5和xa-13的PCR标记，并使用这些来调查一系列的水稻种质。种质资源调查的结果将是有用的选择亲本的育种计划，旨在转移这些白叶枯病抗性基因从一个品种的背景到另一个。
DNA marker-assisted selection was used to pyramid four bacterial blight resistance genes, Xa-4, xa-5, xa-13 and Xa-21. Breeding lines with two, three and four resistance genes were developed and tested for resistance to the bacterial blight pathogen (Xanthonzonas oryzae pv. oryzae). The pyramid lines showed a wider spectrum and a higher level of resistance than lines with only a single gene. To speed up the gene pyramiding process and to facilitate future marker-aided selection, we developed PCR markers for the two recessive genes, xa-5 and xa-13, and used these to survey a range of rice germplasm. The results of the germplasm survey will be useful for the selection of parents in breeding programs aimed at transferring these bacterial blight resistance genes from one varietal background to another.