Effects of organic cations on the gram-negative cell wall and their bactericidal activity with ethylenediaminetetra-acetate and surface active agents.
Effects of organic cations on the gram-negative cell wall and their bactericidal activity with ethylenediaminetetra-acetate and surface active agents.
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有机阳离子对革兰氏阴性细胞壁的影响及其与乙二胺四乙酸和表面活性剂的杀菌活性。
DOI：
10.1099/00221287-48-3-391
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发表时间：
1967
期刊：
影响因子：
--
通讯作者：
J. G. Voss
中科院分区：
文献类型：
--
作者：
J. G. Voss
摘要：当tris缓冲液中溶菌酶和乙二胺四乙酸（EDTA）破坏革兰氏阴性细胞壁时，tris作为有机阳离子发挥积极作用。其他有机阳离子，如脂肪胺和季铵化合物，在修饰细胞壁和使其渗透到其他溶质方面比tris更有效。最有效的有机阳离子有一个C12到C16烷基链。pH10时，EDTA为100/℃。N，N-二甲基十二烷基胺（DDA） 2.5°mg ./ml。对大肠杆菌的细胞壁进行修饰，使其在20°mU/g./ml下的杀伤率达到99.99%以上。两性离子表面活性剂，2-羟基-3-（二甲基十六烷基铵）丙烷-1-磺酸盐（HAPS），在37°dG下，在10分钟内。在这些浓度下，单个化合物的杀菌效果是无效的。另外四种革兰氏阴性菌也同样容易被该系统杀死。EDTA和DDA一起可以去除革兰氏阴性细胞壁的表面成分。单独使用DDA和其他有机阳离子去除细胞壁上的体细胞抗原。
SUMMARY: When Gram-negative cell walls were damaged by lysozyme and ethylene-diaminetetra-acetic acid (EDTA) in tris buffer, tris played an active role through its action as an organic cation. Other organic cations such as aliphatic amines and quaternary ammonium compounds were more effective than tris in modifying the cell wall and making it permeable to other solutes. The most effective organic cations had one C12 to C16 alkyl chain. At pH10, EDTA 100/°mUg./ml and N,N-dimethyldodecylamine (DDA) 2.5 °mUg./ml. modified the cell wall of Escherichia coli so as to permit a more than 99.99% kill by 20 °mU/g./ml. of a zwitterionic surface active agent, 2-hydroxy-3-(dimethylhexadecylammonio) propane-1-sulphonate (HAPS), in 10 min. at 37°dG. At these concentrations, the individual compounds were bactericidally ineffective. Four other Gram-negative species were shown to be similarly susceptible to killing by this system. Together, EDTA and DDA appeared to remove surface components of the Gram-negative cell wall. Alone, DDA and other organic cations removed somatic antigens from the cell wall.