Repression of IGF-I-induced osteoblast migration by (-)-epigallocatechin gallate through p44/p42 MAP kinase signaling.
Repression of IGF-I-induced osteoblast migration by (-)-epigallocatechin gallate through p44/p42 MAP kinase signaling.
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DOI：
10.3892/br.2018.1140
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发表时间：
2018-08
影响因子：
2.3
通讯作者：
Tetsu Kawabata;H. Tokuda;Go Sakai;Kazuhiko Fujita;R. Matsushima-Nishiwaki;T. Otsuka;O. Kozawa
中科院分区：
文献类型：
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作者：
Tetsu Kawabata;H. Tokuda;Go Sakai;Kazuhiko Fujita;R. Matsushima-Nishiwaki;T. Otsuka;O. Kozawa
饮料中的多酚化合物可能对预防骨质疏松症有好处。已有研究表明，胰岛素样生长因子-I(IGF-I)可刺激成骨细胞迁移。在本研究中，研究了咖啡中的主要多酚绿原酸和绿茶中的主要多酚表没食子儿茶素没食子酸酯(EGCG)是否会影响IGF-I刺激的成骨细胞样MC3T3-E1细胞的迁移。通过Transwell细胞迁移和伤口愈合实验评价IGF-I刺激的成骨细胞迁移可被EGCG抑制，但不能被绿原酸抑制。IGF-I可诱导p44/p42丝裂原活化蛋白(MAP)、p70S6和Akt的磷酸化。MAPK1/2抑制剂PD98059和Akt抑制剂Dguelin可抑制IGF-I诱导的细胞迁移，但p70S6激酶上游激酶的抑制剂雷帕霉素不能抑制IGF-I诱导的细胞迁移。EGCG可减弱IGF-I诱导的p44/p42蛋白激酶的磷酸化，但不影响Akt。综上所述，本研究结果提示，EGCG通过p44/p42 MAP激酶抑制IGF-I诱导的成骨细胞迁移。
Polyphenolic compounds in beverages may have benefits in the prevention of osteoporosis. It has been demonstrated previously that insulin-like growth factor-I (IGF-I) could stimulate the migration of osteoblasts. In the present study, it was investigated whether chlorogenic acid, a major polyphenol in coffee, and (-)-epigallocatechin gallate (EGCG), a major polyphenol in green tea, could affect this IGF-I-stimulated migration of osteoblast-like MC3T3-E1 cells. The IGF-I-stimulated osteoblast migration, evaluated by Transwell cell migration and wound-healing assays, was inhibited by EGCG but not chlorogenic acid. IGF-I induced the phosphorylation of p44/p42 mitogen-activated protein (MAP) kinase, p70 S6 kinase and Akt. The IGF-I-induced migration was suppressed by PD98059, a MAP kinase kinase 1/2 inhibitor, and deguelin, an Akt inhibitor, but not rapamycin, an inhibitor of the upstream kinase of p70 S6 kinase (mammalian target of rapamycin). EGCG attenuated the IGF-I-induced phosphorylation of p44/p42 MAP kinase but not Akt. Taken together, the present results suggest that EGCG inhibits IGF-I-induced osteoblast migration via p44/p42 MAP kinase.