A flexible and wearable biosensor for tear glucose measurement
A flexible and wearable biosensor for tear glucose measurement
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DOI：
10.1007/s10544-007-9073-3
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发表时间：
2007-08-01
影响因子：
2.8
通讯作者：
Mitsubayashi, Kohji
中科院分区：
文献类型：
--
作者：
Iguchi, Shigehito;Kudo, Hiroyuki;Mitsubayashi, Kohji
研制了一种柔性、耐磨的电流型葡萄糖传感器。此外，该传感器还用于泪液葡萄糖监测。该传感器通过将GOD固定到柔性氧电极（Pt工作电极和Ag/AgCl反/参比电极）上来构建，该电极使用“软MEMS”技术制造到功能聚合物膜上。在生物仪器的目的，粘合剂不用于构建柔性生物传感器。葡萄糖浓度在0.025-1.475 mmol/L范围内与输出电流呈线性关系，相关系数为0.998。还评估了电流对pH和温度的依赖性。在pH7.0时电流最大，温度升高电流增大。这表明输出电流取决于酶活性。在基本特性研究的基础上，将葡萄糖传感器应用于日本白色兔眼部位泪液中葡萄糖的测量。通过口服葡萄糖引起的泪液葡萄糖水平的变化被监测为附接在眼睛部位上的传感器的电流变化。在这项研究中，泪液葡萄糖水平从0.16到0.46 mmol/l不等。虽然对于血糖水平存在几十分钟的延迟，但认为可以使用柔性生物传感器实现无创连续葡萄糖监测。
A flexible and wearable amperometoric glucose sensor was fabricated and tested. Also, the sensor was utilized to tear glucose monitoring. The sensor was constructed by immobilizing GOD onto a flexible oxygen electrode (Pt working electrode and Ag/AgCl counter/reference electrode), which was fabricated using "Soft-MEMS" techniques onto a functional polymer membrane. In purpose of bioinstrumentation, adhesive agents were not used for constructing the flexible biosensor. Linear relationship between glucose concentration and output current was obtained in a range of 0.025-1.475 mmol/l, with a correlation coefficient of 0.998. Current dependences on pH and temperature were also evaluated. The current was largest at pH 7.0 and the current increased when temperature increased. This indicates that the output current depends on enzyme activity. Based on the basic characteristics investigation, the glucose sensor was applied to measurement of glucose in tear fluids on an eye site of a Japan white rabbit. The change of tear glucose level induced by oral-administration of glucose was monitored as a current change of the sensor attached on the eye site. In this investigation, the tear glucose level varied from 0.16 to 0.46 mmol/l. Although there was a delay of several tens of minutes towards blood sugar level, it is considered to be possible that non-invasive continuous glucose monitoring can be realized using the flexible biosensor.