Static cytofluorometry and fluorescence morphology of mitochondria and DNA in proliferating fibroblasts
Static cytofluorometry and fluorescence morphology of mitochondria and DNA in proliferating fibroblasts
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DOI：
10.3109/10520299609117135
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发表时间：
1996-03-01
影响因子：
1.6
通讯作者：
Pippia, P
中科院分区：
文献类型：
--
作者：
Barni, S;Sciola, L;Pippia, P
用静态细胞荧光法和荧光显微镜观察了人成纤维细胞在细胞周期（G（0）/G（1）、S、G（2））各期线粒体的形态、分布和含量。用Hoechst 33342和DiOC（6）标记DNA和线粒体，对培养细胞进行形态细胞化学观察。染色方式是基于线粒体标记的稳定性。G（1）至早期S期以丝状线粒体的存在为特征，但有丝分裂后早期除外。在S、G（2）和有丝分裂后期，线粒体质量达到最大值，线粒体变短而增多。在有丝分裂后期，线粒体通过胞质桥分布在子细胞之间。
The shape, distribution, and content of mitochondria in individual cells were examined during the cell cycle phases (G(0)/G(1), S, G(2) mitosis) in living human fibroblasts by static cytofluorometry and fluorescence microscopy, The morphocytochemical evaluations were performed in cell cultures submitted to double supravital fluorochrome staining with Hoechst 33342 and DiOC(6) to label DNA and mitochondria, respectively. The staining modalities were based on the stability of mitochondrial labeling, The G(1) to early S phases were characterized by the presence of filamentous mitochondria, except during the early postmitotic period. During late S, G(2), and mitotic phases, mitochondrial mass reached its highest value and mitochondria became short and numerous, During the last stage of mitosis, mitochondria were distributed among daughter cells through a cytoplasmic bridge.