Identification of S-genotypes of four cultivars and sequence analyses of S_(31)-RNase in Eriobotrya Lindl.
Identification of S-genotypes of four cultivars and sequence analyses of S_(31)-RNase in Eriobotrya Lindl.
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作者：
Y. Qi
【目的】确定枇杷‘贵妃’、枇杷‘贵妃’等4个中国枇杷品种的S基因型。粳稻‘黄米’，E. 【方法】利用已知苹果S-RNA酶保守序列设计的两对引物，对4个中国枇杷品种的基因组DNA进行PCR扩增。然后对PCR产物进行克隆和测序，并利用Blast和Bioedit对扩增片段进行同源性比对和序列分析。 【结果】本研究从4个中国枇杷品种中分离到4个S等位基因，分别为S2、S5、S6和S31，其中S31为从粳稻‘软条白沙’品种中克隆的新S基因，登录号为基因库中的 KC131133。与苹果S-RNases相同，这些克隆的S-RNases比对具有1个高变区（HV）和4个保守区（C2、C3、RC4和C5）。【结论】4个供试品种的S基因型鉴定如下：粳稻‘贵妃’（S2-S6）、粳稻‘贵妃’（S2-S6）、粳稻‘贵妃’（S2-S6）、E.粳稻‘黄米’(S2-S5)，E．粳稻‘早红’(S5-S6)，E．粳稻‘软条白沙’(S6-S31)。
【Objective】The objective of this study is to determine the S-genotypes of four Chinese loquat cultivars,including Eriobotrya japonica‘Guifei',E. japonica‘Huangmi',E. japonica‘Zaohong',E.japonica‘Ruantiaobaisha',to provide the scientific basis for pollinizer selection in cultivation and parents selection in breeding.【Method】Using two pairs of primers which designed from conserved sequences of known apple S-RNases,PCR amplification was carried out for genomic DNAs of four Chinese loquat cultivars. Then after cloning and sequencing of PCR products,and the homology blast and sequence analysis of these amplified fragments were carried out by Blast and Bioedit.【Result】In this study,four S alleles were separated from the four Chinese loquat cultivars,namely S2,S5,S6 and S31,and the S31 was a new S-gene cloned from E. japonica‘Ruantiaobaisha'cultivar and its accession number was KC131133 in Gene Bank. Same as the apple S-RNases,the alignment of these cloned S-RNases had one identified hypervariable region(HV) and four conserved regions(C2,C3,RC4 and C5).【Conclusion】The S-genotypes of the four tested cultivars were identified as follows: E. japonica‘Guifei'(S2- S6),E. japonica‘Huangmi'(S2-S5),E. japonica‘Zaohong'(S5-S6),E. japonica‘Ruantiaobaisha'(S6-S31).