INDUCTION OF THE LYTIC VIRAL CYCLE IN EPSTEIN-BARR-VIRUS CARRYING BURKITT-LYMPHOMA LINES IS ACCOMPANIED BY INCREASED EXPRESSION OF MAJOR HISTOCOMPATIBILITY COMPLEX-MOLECULES
INDUCTION OF THE LYTIC VIRAL CYCLE IN EPSTEIN-BARR-VIRUS CARRYING BURKITT-LYMPHOMA LINES IS ACCOMPANIED BY INCREASED EXPRESSION OF MAJOR HISTOCOMPATIBILITY COMPLEX-MOLECULES
复制标题
DOI：
10.1016/0165-2478(93)90008-p
复制
发表时间：
1993-11-01
影响因子：
4.4
通讯作者：
KLEIN, E
中科院分区：
文献类型：
--
作者：
DIRENZO, L;AVILACARINO, J;KLEIN, E
6株携带eb病毒（EBV）基因组的伯基特淋巴瘤（BL）培养物（P3HR-1、Raji、Akata、Daudi、Rael和Jijoye）被诱导进入裂解周期。根据已知的对这些细胞系的诱导能力，分别使用Phorbol esther （TPA）、n-丁酸盐、5-氮杂胞苷（5AzaC）或抗- igg。在一定比例的细胞中出现病毒早期抗原（EA）的同时，主要组织相容性复合体（MHC） II类抗原的表达在培养中增加。P3HR-1和Raji细胞I类表达也增加。MHC表达的增强与诱导效率相关，并且只需要病毒裂解周期的早期事件。TPA加正丁酸盐或5AzaC治疗3种ebv阴性淋巴瘤系（BJAB、Ramos和BL41）对MHC表达没有影响。此外，在体外感染后携带EBV基因组且不能被诱导进行病毒裂解周期的BL系在诱导剂处理后没有改变MHC表达。在混合培养中，与未处理的细胞相比，MHC表达升高的诱导细胞的异体刺激能力更强。
Six Epstein Barr virus (EBV) genome-carrying Burkitt lymphoma (BL) cultures (P3HR-1, Raji, Akata, Daudi, Rael and Jijoye) were induced to enter the lytic cycle. Phorbol esther (TPA), n-butyrate, 5-azacytidine (5AzaC) or anti-IgG were used according to their known inducing capacity on these cell lines. Concomitantly with the appearance of the viral early antigens (EA) in a proportion of cells, the expression of major histocompatibility complex (MHC) class II antigens increased in the cultures. On P3HR-1 and Raji cells class I expression also increased.The enhancement of MHC expression correlated with the efficiency of induction and required only an early event of the viral lytic cycle. Treatment of 3 EBV-negative lymphoma lines (BJAB, Ramos and BL41) with TPA plus n-butyrate or 5AzaC did not influence MHC expression. Moreover, BL lines which carry the EBV genome after having been infected in vitro and which cannot be induced for the viral lytic cycle did not change MHC expression after treatment with theinducing agents.In mixed cultures the allo-stimulatory capacity of induced cells with elevated MHC expression was stronger compared to the untreated ones.