In vitro evaluation of photon and carbon ion radiotherapy in combination with chemotherapy in glioblastoma cells.
In vitro evaluation of photon and carbon ion radiotherapy in combination with chemotherapy in glioblastoma cells.
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DOI：
10.1186/1748-717x-7-9
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发表时间：
2012-01-27
期刊：
影响因子：
--
通讯作者：
Weber KJ
中科院分区：
文献类型：
--
作者：
Combs SE;Zipp L;Rieken S;Habermehl D;Brons S;Winter M;Haberer T;Debus J;Weber KJ
目的：评价碳离子放化疗对胶质母细胞瘤细胞的细胞毒作用。对人胶质母细胞瘤(GBM)细胞株U87进行2Gy4Gy6Gy3个剂量的光子放射治疗。同样，用碳离子照射，单次碳剂量分别为0.125、0.5、2和3Gy.分别用喜树碱、吉西他滨、紫杉醇和顺铂四种化疗药物进行单独和联合实验。采用代表放射生物学金标准的克隆性生长试验评价单次和双次处理对细胞活性的影响。根据生存水平和剂量的不同，碳离子的相对生物效应在3.3到3.9之间。所有化疗药物均呈明显的剂量-反应关系。以其特有的浓度。在随后的联合实验中，选择了两个剂量水平，导致细胞存活率的低水平和中等水平降低。联合实验表明，相加效应与药物的作用机制无关。联合碳离子放射治疗时，紫杉醇和喜树碱的细胞毒作用最为显著。综上所述，碳离子放射治疗与不同作用机制的化疗相结合表现出相加效应。最主要的作用是紫杉醇，其次是喜树碱，从以前发表的研究中可以预料到这一点。这些数据为进一步的联合实验以及联合治疗的临床研究提供了重要的放射生物学基础。
To evaluate the cytotoxic effect of carbon ion radiotherapy and chemotherapy in glioblastoma cells in vitro. The human glioblastoma (GBM) cell line U87 was irradiated with photon radiotherapy (RT) doses of 2 Gy, 4 Gy and 6 Gy. Likewise, irradiation with carbon ions was performed with single carbon doses of 0.125, 0.5, 2 and 3 Gy. Four chemotherapeutic substances, camptothecin, gemcitabine, paclitaxel and cisplatinum, were used for single and combination experiments. The assessment of the effect of single and double treatment on cell viability was performed using the clonogenic growth assay representing the radiobiological gold standard. The RBE of carbon ions ranges between 3.3 and 3.9 depending on survival level and dose. All chemotherapeutic substances showed a clear does-response relationhips. in their characteristic concentrations. For subsequent combination experiments, two dose levels leading to low and medium reduction of cell survival were chosen. Combination experiments showed additive effects independently of the drugs' mechanisms of action. Paclitaxel and campthothecin demonstrated the most prominent cytotoxic effect in combination with carbon ion radiotherapy. In conclusion, combination of carbon ion radiotherapy with chemotherapies of different mechanisms of action demonstrates additive effects. The most dominant effect was produced by paclitaxel, followed by camptothecin, as espected from previously published work. The present data serve as an important radiobiological basis for further combination experiments, as well as clinical studies on combination treatments.