Microbial community structure of the bovine rumen as affected by feeding cashew nut shell liquid, a methane‐inhibiting and propionate‐enhancing agent.
Microbial community structure of the bovine rumen as affected by feeding cashew nut shell liquid, a methane‐inhibiting and propionate‐enhancing agent.
复制标题
饲喂腰果壳液（一种甲烷抑制剂和丙酸增强剂）对牛瘤胃微生物群落结构的影响。
DOI：
10.1111/asj.13503
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发表时间：
2021
影响因子：
2
通讯作者：
Y.
中科院分区：
文献类型：
--
作者：
Su;C.;Shinkai;T.;Miyazawa;N.;Mitsumori;M.;Enishi;O.;Nagashima;K.;Koike,S.;Kobayashi;Y.
通过分析针对16S rRNA基因、甲基辅酶还原酶A编码基因(MCRA)及其各自转录产物的克隆文库，研究了添加腰果壳液(CNSL)对牛瘤胃细菌和古菌群落的影响。瘤胃样本采集自三头非哺乳期奶牛，饲喂干草和精料日粮，添加或不添加CNSL。构建了用于瘤胃微生物群落研究的DNA文库和互补DNA文库。为了更全面地了解微生物群落结构的变化，还进行了MiSeq分析。添加CNSL后，细菌和古菌群落的可操作分类单位(OTU)数和多样性指数均降低。属于变形杆菌的细菌OTUS，包括弧菌，在CNSL的摄食过程中出现频率较高，尤其是在cDNA文库中。产甲烷古生菌群落以甲烷微生物为主。在MiSeq数据中也观察到细菌群落的变化，表明CNSL增加了苏云金弧菌和其他已知参与丙酸生产的属的比例。产甲烷古生菌群落也发生了甲烷平台菌的增加和甲烷短杆菌的减少。综上所述，这些结果表明，在饲喂CNSL后，瘤胃群落发生了显著的变化，不仅是细菌，还有产甲烷菌。
The effect of cashew nut shell liquid (CNSL) feeding on bacterial and archaeal community of the bovine rumen was investigated by analyzing clone libraries targeting 16S rRNA genes, methyl‐coenzyme reductase A‐encoding genes (mcrA), and their respective transcripts. Rumen samples were collected from three non‐lactating cows fed on a hay and concentrate diet with or without CNSL supplementation. DNA and complementary DNA (cDNA) libraries were generated for investigating rumen microbial communities. MiSeq analysis also was performed to understand more comprehensively the changes in the microbial community structures. Following CNSL supplementation, the number of operational taxonomical unit (OTU) and diversity indices of bacterial and archaeal community were decreased. Bacterial OTUs belonging toProteobacteria, includingSuccinivibrio, occurred at a higher frequency with CNSL feeding, especially in cDNA libraries. The methanogenic archaeal community became dominated byMethanomicrobium. A bacterial community shift also was observed in the MiSeq data, indicating that CNSL increased the proportion ofSuccinivibrioand other genera known to be involved in propionate production. Methanogenic archaeal community shifts to increaseMethanoplanusand to decreaseMethanobrevibacteralso were observed. Together, these results imply the occurrence of significant changes in rumen communities, not only for bacteria but also for methanogens, following CNSL feeding.