Association of the-173G/C polymorphism of the macrophage migration inhibitory factor gene with ulcerative colitis
Association of the-173G/C polymorphism of the macrophage migration inhibitory factor gene with ulcerative colitis
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DOI：
10.1007/s00535-003-1284-7
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发表时间：
2004-03-01
影响因子：
6.3
通讯作者：
Hinoda, Y
中科院分区：
文献类型：
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作者：
Nohara, H;Okayama, N;Hinoda, Y
背景巨噬细胞移动抑制因子（MIF）是一种促炎细胞因子，并已被证明参与慢性小鼠结肠炎的发展。在MIF基因的-173 G/C多态性中，C的存在产生激活蛋白4的结合基序。本研究探讨了这种多态性与溃疡性结肠炎（UC）的关联。方法.采用四引物聚合酶链反应（PCR）方法对438名健康志愿者和221例UC患者的659份DNA标本进行基因分型。对病例组和对照组的基因型分布以及患者基因型与临床参数的关系进行统计学分析。结果UC患者和健康对照组的基因型分布差异无统计学意义。然而，当通过Fisher精确检验评估UC患者中C/C基因型与临床参数的关系时，发现全结肠炎型患者中C/C基因型的频率高于局限于远端或左侧结肠的其他类型患者（比值比[OR]，10.781; 95%置信区间[CI]，1.342-86.619; P = 0.0074）。结论.这些数据表明，在日本人群中，MIF-173 G/C多态性可能与UC的疾病程度相关。
Background. Macrophage migration inhibitory factor (MIF) is a proinflammatory cytokine and has been shown to be involved in the development of chronic murine colitis. In the -173 G/C polymorphism of the MIF gene, the presence of C creates the binding motif of activator protein 4. This study explored the association of this polymorphism with ulcerative colitis (UC). Methods. Genotyping was carried out, with a tetra-primer polymerase chain reaction (PCR) method, for 659 DNA specimens from 438 healthy volunteers and 221 patients with UC. Genotype distribution between cases and controls and the association of patients' genotypes with clinical parameters were statistically evaluated. Results. No significant difference in genotype distribution was found between UC patients and healthy controls. However, when the relation of the C/C genotype to clinical parameters in UC patients was evaluated by Fisher's exact test, it was found that the frequency of the C/C genotype was higher in patients with pancolitis type than in those with other types restricted to the distal or left-sided colon (odds ratio [OR], 10.781; 95% confidence interval [CI], 1.342-86.619; P = 0.0074). Conclusions. These data suggest that the MIF -173 G/C polymorphism may be related to the extent of disease in UC in a Japanese population.