[Fingerprints of Rhizoma Coptidis from Shizhu by HPLC].
[Fingerprints of Rhizoma Coptidis from Shizhu by HPLC].
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发表时间：
2006-07
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通讯作者：
Xiao-ping Huang;Long-yun Li;Xian-you Qu;Guang-lin Cui
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作者：
Xiao-ping Huang;Long-yun Li;Xian-you Qu;Guang-lin Cui
目的建立重庆石柱地区黄连药材的高效液相色谱质量控制方法。方法采用Waters仪器对石柱黄连进行HPLC指纹图谱分析。采用Diamonsil C18色谱柱梯度洗脱，乙腈-0.05 mol/L KH2PO4 （pH3 with H3PO4）洗脱，流速0.8 m/min。色谱柱温度25℃，紫外检测波长270nm。结果从10批黄连药材中建立了16个特征峰的HPLC指纹图谱。通过对保留时间和化学标准品在线紫外光谱的比较，峰11、12、13、14和15分别为Epiberberine、Jatrorrhizine、Coptisine、Palmatine和Berberine。结论黄连药材的高效液相色谱指纹图谱具有较高的特异性，可用于黄连药材的质量控制。
OBJECTIVE To establish a sensitive and specific HPLC method for the quality control of Rhizoma Coptidis collected from shizhu in chongqing. METHODS The HPLC fingerprints of Rhizoma Coptidis from shizhu were obtained from Waters instrument. The methods was performed on a Diamonsil C18 column gradient eluted with acetonitrile-0.05 mol/L KH2PO4 (pH3 with H3PO4) at the flow rate of 0.8 m/min. The temperature of column was 25 degrees C and the UV detection wavelength was 270nm. RESULTS The HPLC fingerprint of Rhizoma Coptis, showing 16 characteristic peaks, was established from 10 lots of Rhizoma Coptis. By comparision of the retention time and the on-line UV spectra of chemical standards, peak 11, 12, 13, 14 and 15 were identified as Epiberberine, Jatrorrhizine, Coptisine, Palmatine and Berberine. CONCLUSION The HPLC fingerprint of Rhizoma Coptidis with high specificity can be used to control its quality.