Detection of aneuploidy in human spermatozoa of normal semen donors by fluorescence in situ hybridization
Detection of aneuploidy in human spermatozoa of normal semen donors by fluorescence in situ hybridization
复制标题
DOI：
10.2307/3432835
复制
发表时间：
1996-05-01
影响因子：
10.4
通讯作者：
Mykkanen, J
中科院分区：
文献类型：
--
作者：
Lahdetie, J;AjosenpaaSaari, M;Mykkanen, J
我们研究了人类精子从24个正常的，健康的未暴露的男子，其中18人是精液捐献者在精子库在图尔库，使用荧光原位杂交与两个染色体特异性探针。评估了可能与年龄相关的非整倍体频率增加。对每个个体的一万个精子进行超倍体评分，即，二体和二倍体细胞。总杂交效率为98.8%。精子中有两条1号染色体信号的频率为11.+/-. 5.2/10,000.具有两个7号染色体信号的精子的频率为6.4+/-3.9/10，000。二倍体存在于15.0+/-8.9/10，000个精子中。个体间变异较大。未观察到供体年龄（范围=20-46岁）与超倍体精子频率之间的统计学显著相关性。这些结果提供了未暴露男性超倍体精子发生率的背景信息，并鼓励使用这种新技术对暴露于潜在非整倍体生成剂的男性进行遗传效应的未来研究。
We have studied human spermatozoa from 24 normal, healthy unexposed men, 18 of whom were semen donors at the Sperm Bank in Turku, using multicolor fluorescence in situ hybridization with two chromosome-specific probes. The possible age-related increase in aneuploidy frequencies was assessed. Ten thousand spermatozoa were scored per individual for the presence of hyperploid, i.e., disomic and diploid, cells. The overall hybridization efficiency was 98.8%. The frequency of spermatozoa with two chromosome 1 signals was 11.+/-5.2/10,000. The frequency of spermatozoa with two chromosome 7 signals was 6.4+/-3.9/10,000. Diploidy was present in 15.0+/-8.9/10,000 spermatozoa. Interindividual variation was quite large. No statistically significant correlation between age of the donors (range=20-46 years) and the frequency of hyperploid spermatozoa was observed. The results give background information on the incidence of hyperploid spermatozoa in unexposed men and encourage the use of this novel technique for future studies on genetic effects in men exposed to potentially aneuploidogenic agents.