Identification and Physical Mapping of New PCR-Based Markers Specific for the Long Arm of Rye (Secale cereale L.) Chromosome 6
Identification and Physical Mapping of New PCR-Based Markers Specific for the Long Arm of Rye (Secale cereale L.) Chromosome 6
复制标题
黑麦长臂 6 号染色体特异的新型 PCR 标记的鉴定和物理作图
DOI：
10.1016/j.jgg.2015.11.005
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发表时间：
2016-04-20
影响因子：
5.9
通讯作者：
Fu, Shulan
中科院分区：
文献类型：
--
作者：
Li, Meng;Tang, Zongxiang;Fu, Shulan
为了有效地利用黑麦6号染色体长臂（6 RL臂）上的优良基因进行小麦育种，需要精确快速地鉴定小麦背景中的6 RL染色质。基于PCR技术的6 RL特异性标记可用于小麦育种中6 RL基因的检测。然而，仅开发了有限数量的6 RL特异性标记物。在本研究中，使用特定长度扩增片段测序（SLAF-seq）技术鉴定了300个新的基于PCR的6 RL特异性标记，并使用6 R和6 RL缺失线进一步物理定位到6 RL臂上的四个区域。有趣的是，300个标记中的127个物理定位于从2.3和2.5之间的位点到端粒的区域，与白粉病抗性基因定位的区域相同。此外，300个标记中有95个表现出多态性，可用于研究黑麦6 RL臂的多样性。本研究所开发的标记可用于小麦背景中6 RL基因片段的鉴定，促进6 RL基因在小麦育种中的利用。
To effectively use elite genes on the long arm of rye chromosome 6 (the 6RL arm) in wheat breeding programs, precise and fast identification of 6RL chromatin in wheat backgrounds is necessary. PCR-based 6RL-specific markers can facilitate the detection of elite genes on 6RL in wheat breeding. However, only a limited number of 6RL-specific markers have been developed. In the present study, 300 new PCR-based 6RL-specific markers were identified using specific length amplified fragment sequencing (SLAF-seq) technology, and were further physically mapped to four regions on the 6RL arm using 6R and 6RL deletion lines. Interestingly, 127 of the 300 markers were physically localized to a region from the site between 2.3 and 2.5 to the telomere, the same region where the powdery mildew resistance gene was mapped. In addition, 95 of the 300 markers exhibit polymorphisms, which can be used to investigate the diversity of rye 6RL arms. The markers developed in this study can be used to identify given segments of 6RL in wheat backgrounds and accelerate the utilization of elite genes on 6RL in wheat breeding.