Laryngeal electromyographic, cardiovascular, and respiratory effects of neuropeptide injections into the nucleus tractus solitarius of rats.
Laryngeal electromyographic, cardiovascular, and respiratory effects of neuropeptide injections into the nucleus tractus solitarius of rats.
复制标题
神经肽注射到大鼠孤束核中对喉肌电图、心血管和呼吸的影响。
DOI：
10.1177/000348940411301007
复制
发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Wang,DeQiang
中科院分区：
文献类型：
--
作者：
Bauman,NancyM;Wang,DeQiang
识别介导喉内收肌活动的中枢神经递质可能有助于控制婴儿喉痉挛或明显危及生命的事件中发生的病理性喉内收。我们研究了向大鼠孤束核（NTS）（喉部的主要感觉中继中心）注射神经递质对环甲腺（CT）和甲杓肌（TA）肌电图（EMG）以及心血管参数的影响。将20纳升血管活性肠肽（VIP；2.5 pmol）、神经激肽B（NKB，16 pmol）、降钙素基因相关肽（3.0 pmol）、神经激肽A（NKA；35 pmol）或人工脑脊液（对照溶液）立体定向注射到NTS和对照核区域 在 24 只成熟、麻醉的 Sprague-Dawley 大鼠中进行的 119 项研究中，发现了 gracilis。比较膈肌、CT 和 TA EMG 活动以及血压 (BP) 的变化。注射部位经过组织学验证。将 VIP 和 NKB 注射到 NTS 区域（而非股薄核）会引起危及生命的变化，包括呼吸暂停、血压显着下降 (p < .05)，以及 CT 和 TA 内收肌的 EMG 活动增加，从持续收缩到吸气期间轻度阶段性增加。降钙素基因相关肽注射也可降低血压 (p < .05)，但仅导致 CT 和 TA EMG 活性轻度增加。 NKA 和对照注射到 NTS 中不会改变呼吸、血压、CT 或 TA EMG 活性。 VIP 和 NKB 可能在调节 CT 和 TA 肌肉的 EMG 活动中发挥重要作用。该信息可能有助于评估中枢反射活动的药理学目标，以控制危及生命的喉内收。
Identifying central neurotransmitters that mediate laryngeal adductor activity may aid in managing pathological laryngeal adduction as occurs in laryngospasm or apparent life-threatening events in infants. We studied the effect on cricothyroid (CT) and thyroarytenoid (TA) electromyography (EMG) and on cardiovascular parameters of neurotransmitter injections into the rat nucleus tractus solitarius (NTS), the primary sensory relay center of the larynx. Twenty nanoliters of vasoactive intestinal peptide (VIP; 2.5 pmol), neurokinin B (NKB, 16 pmol), calcitonin gene-related peptide (3.0 pmol), neurokinin A (NKA; 35 pmol), or artificial cerebrospinal fluid (control solution) was stereotactically injected into the region of the NTS and the control nucleus gracilis in 119 studies performed in 24 mature, anesthetized Sprague-Dawley rats. Changes in diaphragm, CT, and TA EMG activity and blood pressure (BP) were compared. Injection sites were verified histologically. Injections of both VIP and NKB into the region of the NTS, but not the nucleus gracilis, induced life-threatening changes, including apnea, a marked decline in BP (p < .05), and increases in EMG activity of the CT and TA adductor muscles ranging from sustained contraction to mild phasic increases during inspiration. Calcitonin gene-related peptide injection also decreased BP (p < .05), but caused only mild increases in CT and TA EMG activity. NKA and control injections into the NTS did not alter respiration, BP, or CT or TA EMG activity. VIP and NKB may play important roles in modulating EMG activity of the CT and TA muscles. This information may prove useful in evaluating pharmacological targets of central reflex activity to manage life-threatening laryngeal adduction.