The Arabidopsis minE mutation causes new plastid and FtsZ1 localization phenotypes in the leaf epidermis.
The Arabidopsis minE mutation causes new plastid and FtsZ1 localization phenotypes in the leaf epidermis.
复制标题
PtAGL24 的表征和表达分析，PtAGL24 是来自枳（Poncirus trifoIlata L. Raf.）的短营养期/无性恋样 24 (SVP/AGL24) 型 MADS-Box 基因
DOI：
10.3389/fpls.2015.00823
复制
发表时间：
2015
影响因子：
5.6
通讯作者：
Itoh RD
中科院分区：
文献类型：
--
作者：
Fujiwara MT;Kojo KH;Kazama Y;Sasaki S;Abe T;Itoh RD
植物叶表皮细胞中的质体被认为是未成熟的叶绿体，与叶肉叶绿体一样，经历二次分裂。虽然叶绿体通常被用来研究叶绿体的分裂，但最近的研究表明，叶绿体分裂存在依赖于组织或叶绿体类型的调控。在这里，我们报道了叶绿体分裂相关蛋白AtFtsZ1-1在叶绿体分裂位点决定基因AtMinE1中突变的拟南芥突变体的叶绿体和叶基质的详细形态，以及叶绿体分裂相关蛋白AtFtsZ1-1的胞内定位。在atminE1中，叶绿体的大小和形状变化很大，这与atminE1叶肉细胞中观察到的叶绿体表型形成了鲜明的对比。具体地说，atminE1表皮体偶尔会表现出葡萄状的形态，这是一种由叶绿体分裂突变诱导的新表型。对含有AtMinE1启动子：：AtMinE1-黄色荧光蛋白融合基因的atminE1转基因系的观察证实了AtMinE1在叶表皮中的表达和可塑性定位。进一步的研究表明，FtsZ1环介导的叶绿体和球茎的收缩导致了atminE1表皮中的叶绿体多型性。这些结果表明，单个叶绿体分裂突变可以对表皮体的形态产生显著影响，从而意味着叶绿体和叶绿体中的叶绿体分裂和形态发生受到不同的调控。
Plastids in the leaf epidermal cells of plants are regarded as immature chloroplasts that, like mesophyll chloroplasts, undergo binary fission. While mesophyll chloroplasts have generally been used to study plastid division, recent studies have suggested the presence of tissue- or plastid type-dependent regulation of plastid division. Here, we report the detailed morphology of plastids and their stromules, and the intraplastidic localization of the chloroplast division-related protein AtFtsZ1-1, in the leaf epidermis of an Arabidopsis mutant that harbors a mutation in the chloroplast division site determinant gene AtMinE1. In atminE1, the size and shape of epidermal plastids varied widely, which contrasts with the plastid phenotype observed in atminE1 mesophyll cells. In particular, atminE1 epidermal plastids occasionally displayed grape-like morphology, a novel phenotype induced by a plastid division mutation. Observation of an atminE1 transgenic line harboring an AtMinE1 promoter::AtMinE1-yellow fluorescent protein fusion gene confirmed the expression and plastidic localization of AtMinE1 in the leaf epidermis. Further examination revealed that constriction of plastids and stromules mediated by the FtsZ1 ring contributed to the plastid pleomorphism in the atminE1 epidermis. These results illustrate that a single plastid division mutation can have dramatic consequences for epidermal plastid morphology, thereby implying that plastid division and morphogenesis are differentially regulated in epidermal and mesophyll plastids.