Contrasting patterns of the 5S and 45S rDNA evolutions in the Byblis liniflora complex (Byblidaceae).
Contrasting patterns of the 5S and 45S rDNA evolutions in the Byblis liniflora complex (Byblidaceae).
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DOI：
10.1007/s10265-010-0366-x
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发表时间：
2011-03
影响因子：
2.8
通讯作者：
Hoshi, Yoshikazu
中科院分区：
文献类型：
--
作者：
Fukushima, Kenji;Imamura, Kaori;Nagano, Katsuya;Hoshi, Yoshikazu
为了阐明亚麻花鬼针草复合体（Byblis liniflora complex，Bybridaceae）核糖体RNA基因（ribosomal RNA gene，rDNA）的进化动态，我们通过荧光原位杂交（fluorescence in situ hybridization，FISH）染色体物理作图和5S rDNA非转录间隔区（5S NTS）和45S rDNA内转录间隔区（ITS）的系统发育分析，对亚麻花鬼针草复合体的5S和45S rDNA基因进行了研究。此外，我们进行了系统发育分析的基础上rbcL和trnK内含子。该复合体可分为2个分支：B.水生生物filifolia和B.盖霍伊湾亚麻属罗里达。虽然该复合体的成员具有保守的对称核型，但它们在染色体rDNA分布模式上有明显的差异。ITS序列分析结果表明，所有类群的ITS序列几乎都是均一的，在体细胞核型中，在染色体的远侧区域经常出现两个或四个45SrDNA阵列。ITS均质化可能是由相对较远的45S rDNA位置引起的。相反，2 - 12个5S rDNA阵列被定位在染色体的近端/间质区域，并且在含有4个或更多阵列的基因组中发现了一些旁系同源的5S-NTS。5S-NTS序列类型特异性FISH分析表明，一些5S rDNA阵列内和之间的序列异质性。基因座间的均一化可能受到它们在染色体上的邻近位置的阻碍。染色体位置可能影响了B中rDNAs的进化动力学。亚麻复合体
To clarify the evolutionary dynamics of ribosomal RNA genes (rDNAs) in the Byblis liniflora complex (Byblidaceae), we investigated the 5S and 45S rDNA genes through (1) chromosomal physical mapping by fluorescence in situ hybridization (FISH) and (2) phylogenetic analyses using the nontranscribed spacer of 5S rDNA (5S-NTS) and the internal transcribed spacer of 45S rDNA (ITS). In addition, we performed phylogenetic analyses based on rbcL and trnK intron. The complex was divided into 2 clades: B. aquatica–B. filifolia and B. guehoi–B. liniflora–B. rorida. Although members of the complex had conservative symmetric karyotypes, they were clearly differentiated on chromosomal rDNA distribution patterns. The sequence data indicated that ITS was almost homogeneous in all taxa in which two or four 45S rDNA arrays were frequently found at distal regions of chromosomes in the somatic karyotype. ITS homogenization could have been prompted by relatively distal 45S rDNA positions. In contrast, 2–12 5S rDNA arrays were mapped onto proximal/interstitial regions of chromosomes, and some paralogous 5S-NTS were found in the genomes harboring 4 or more arrays. 5S-NTS sequence type-specific FISH analysis showed sequence heterogeneity within and between some 5S rDNA arrays. Interlocus homogenization may have been hampered by their proximal location on chromosomes. Chromosomal location may have affected the contrasting evolutionary dynamics of rDNAs in the B. liniflora complex.
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DOI：
10.1111/j.1096-0031.1996.tb00196.x
发表时间：
1994-09-01
期刊：
CLADISTICS-THE INTERNATIONAL JOURNAL OF THE WILLI HENNIG SOCIETY
影响因子：
--
作者：
FARRIS, JS;KALLERSJO, M;BULT, C
通讯作者：
BULT, C
影响因子：
1.6
作者：
Hanson, RE;IslamFaridi, MN;Price, HJ
通讯作者：
Price, HJ
影响因子：
7.4
作者：
Higashiyama, Tetsuya;Inatsugi, Rie;Nakano, Akihiko
通讯作者：
Nakano, Akihiko
影响因子：
2.8
作者：
Adachi, J;Watanabe, K;Kosuge, K
通讯作者：
Kosuge, K
影响因子：
1.1
作者：
Conran, JG;Houben, A;Lowrie, A
通讯作者：
Lowrie, A