Control of cell type proportioning in Dictyostelium discoideum by differentiation-inducing factor as determined by in situ hybridization
Control of cell type proportioning in Dictyostelium discoideum by differentiation-inducing factor as determined by in situ hybridization
复制标题
DOI：
10.1128/ec.3.5.1241-1248.2004
复制
发表时间：
2004-10-01
期刊：
影响因子：
--
通讯作者：
Loomis, WF
中科院分区：
文献类型：
--
作者：
Maruo, T;Sakamoto, H;Loomis, WF
我们已经确定了在Dictyosteelium discoideum蛞蝓的prespore和prestalk区域的比例，通过原位杂交与大量的prespore和prestalk特异性基因。微阵列用于发现以细胞类型特异性方式表达的基因。通过原位杂交验证了54个前孢子特异性基因，其中包括18个先前已被证明是细胞类型特异性的基因。这36个新基因使可用的前孢子标记数量增加了一倍多。在蛞蝓阶段，前孢子基因杂交细胞均匀的后80%的野生型蛞蝓，但杂交后90%的蛞蝓缺乏分泌的烷基苯酮分化诱导因子1（DIF-1）。有一个代偿性的两倍减少，在前柄细胞DIF-无蛞蝓。去除前孢子细胞导致野生型和DIF较少的前片段中的细胞类型转换。因此，DIF-1似乎与其他过程协同作用以建立细胞类型比例。
We have determined the proportions of the prespore and prestalk regions in Dictyostelium discoideum slugs by in situ hybridization with a large number of prespore- and prestalk-specific genes. Microarrays were used to discover genes expressed in a cell type-specific manner. Fifty-four prespore-specific genes were verified by in situ hybridization, including 18 that had been previously shown to be cell type specific. The 36 new genes more than doubles the number of available prespore markers. At the slug stage, the prespore genes hybridized to cells uniformly in the posterior 80% of wild-type slugs but hybridized to the posterior 90% of slugs lacking the secreted alkylphenone differentiation-inducing factor 1 (DIF-1). There was a compensatory twofold decrease in prestalk cells in DIF-less slugs. Removal of prespore cells resulted in cell type conversion in both wild-type and DIF-less anterior fragments. Thus, DIF-1 appears to act in concert with other processes to establish cell type proportions.