Dietary L-leucine and L-alanine supplementation have similar acute effects in the prevention of high-fat diet-induced obesity
Dietary L-leucine and L-alanine supplementation have similar acute effects in the prevention of high-fat diet-induced obesity
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DOI：
10.1007/s00726-012-1363-2
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发表时间：
2013-02-01
期刊：
影响因子：
3.5
通讯作者：
Klaus, Susanne
中科院分区：
文献类型：
--
作者：
Freudenberg, Anne;Petzke, Klaus J.;Klaus, Susanne
高蛋白饮食已被证明可以减轻高脂肪饮食的有害影响，这种效果可以通过饮食中补充L亮氨酸部分模拟。在这里，我们的目的是阐明亮氨酸效应的早期机制和特异性。我们对雄性C57BL/6小鼠进行了为期一周的试验，随机饲喂含有适量(10%w/w，AP)或高(50%w/w，HP)乳清蛋白的半合成高脂饲料，或添加与HP饲料中亮氨酸含量相对应的L-亮氨酸(Leu)或添加等摩尔L-丙氨酸(Ala)。使用计算机控制的监测系统连续监测食物和水的摄入量，并使用定量核磁共振评估身体成分的变化。HP完全阻止了AP诱导的体脂蓄积。亮氨酸和丙氨酸对体脂积聚的影响相似，介于AP和HP之间。对血糖和胰岛素无明显影响。与AP、Leu和ALA相比，HP可降低肝脏三酰甘油含量和肝脏脂肪生成酶基因表达，降低血浆胆固醇水平，再次显示出中间效应。体脂增加和肝脏三酰甘油与总能量摄入量密切相关。饲喂HP能迅速增加总摄水量，总摄水量与总氨基氮摄入量呈显著正相关。我们的结论是，高蛋白饮食对代谢综合征相关性状的积极影响是由于饱腹感的影响，可能与氨基氮的摄入有关，以及随后抑制肝脏脂肪生成的影响，而没有证据表明特定的亮氨酸效应。
High-protein diets have been shown to alleviate detrimental effects of high-fat diets and this effect can be partially mimicked by dietary l-leucine supplementation. Here, we aimed to elucidate the early mechanisms and the specificity of leucine effects. We performed a 1-week trial with male C57BL/6 mice fed ad libitum with semisynthetic high-fat diets containing an adequate (10 % w/w, AP) or high (50 % w/w, HP) amount of whey protein, or supplemented with l-leucine corresponding to the leucine content within the HP diet (Leu) or supplemented with equimolar l-alanine (Ala). Food and water intake were monitored continuously using a computer-controlled monitor system and body composition changes were assessed using quantitative NMR. HP completely prevented the AP-induced accumulation of body fat. Leu and Ala resulted in a similar reduction of body fat accumulation which was intermediate between AP and HP. There were no significant effects on plasma glucose or insulin. Triacylglycerol content and gene expression of lipogenesis enzymes in liver as well as plasma cholesterol were reduced by HP compared to AP with Leu and Ala again showing intermediate effects. Body fat gain and liver triacylglycerols were strongly correlated with total energy intake. Water intake was rapidly increased by HP feeding and total water intake correlated strongly with total amino nitrogen intake. We concluded that the positive effects of high-protein diets on metabolic syndrome associated traits are acutely due to effects on satiety possibly linked to amino nitrogen intake and on the subsequent suppression of liver lipogenesis without evidence for a specific leucine effect.