Effects of epidermal growth factor receptor concentration on tumorigenicity of A431 cells in nude mice.
Effects of epidermal growth factor receptor concentration on tumorigenicity of A431 cells in nude mice.
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发表时间：
1986-09
期刊：
影响因子：
11.2
通讯作者：
J. B. Santon;M. Cronin;C. Macleod;J. Mendelsohn;H. Masui;G. Gill
中科院分区：
文献类型：
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作者：
J. B. Santon;M. Cronin;C. Macleod;J. Mendelsohn;H. Masui;G. Gill
为了验证表皮生长因子（EGF）受体浓度与体内肿瘤生长的关系，我们测量了几种独立分离的A431细胞系在胸腺小鼠体内的生长速率。将具有不同程度EGF受体基因扩增和蛋白表达的A431克隆变异体植入胸腺小鼠体内，测定其实体瘤形成时间和生长速度。这些实验结果表明，EGF受体的基因扩增程度和浓度与这些细胞在宿主动物体内作为实体瘤的生长直接相关。互补DNA杂交分析显示，与切除肿瘤相比，植入细胞中基因扩增和表达的程度没有变化，也没有证据表明在体内存在进一步的基因重排。高浓度的EGF受体似乎能促进肿瘤细胞在体内和体外的生长。
To test the relationship between the concentration of epidermal growth factor (EGF) receptors and tumor growth in vivo, we measured the rate of growth of several independently isolated A431 cell lines in athymic mice. This series of A431 clonal variants with differing extents of EGF receptor gene amplification and protein expression were implanted into athymic mice and the time to solid tumor formation and rate of growth were measured. Results of these experiments indicate that the degree of gene amplification and concentration of EGF receptors are directly correlated with the growth of these cells as solid tumors in host animals. Complementary DNA hybridization analysis revealed no change in the extent of gene amplification and expression in implanted cells versus excised tumors nor any evidence of further gene rearrangement in vivo. A high concentration of EGF receptors appears to facilitate the growth of tumor cells in vivo and in vitro.