ENHANCED NA+-H+ EXCHANGER ACTIVITY AND NHE-1 MESSENGER-RNA EXPRESSION IN LYMPHOCYTES FROM PATIENTS WITH ESSENTIAL-HYPERTENSION
ENHANCED NA+-H+ EXCHANGER ACTIVITY AND NHE-1 MESSENGER-RNA EXPRESSION IN LYMPHOCYTES FROM PATIENTS WITH ESSENTIAL-HYPERTENSION
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DOI：
10.1161/01.hyp.25.3.356
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发表时间：
1995-03-01
期刊：
影响因子：
8.3
通讯作者：
DIEZ, J
中科院分区：
文献类型：
--
作者：
GARCIANDIA, A;LOPEZ, R;DIEZ, J
It has been demonstrated that the activity of the sodium-proton exchanger (NHE-1 isoform) is increased in lymphocytes and other blood cells from patients with essential hypertension. In the present study, we investigated whether an increased level of NHE-1-specific mRNA in lymphocytes from patients with essential hypertension would explain the enhanced transport activity. Twenty-two hypertensive patients and 21 normotensive subjects were studied. Basal cytosolic pH was measured by the pH-sensitive fluorescent probe 2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein. Maximal sodium-proton exchange activity was determined by acidifying cell pH and measuring the initial rate of the net sodium-dependent proton efflux driven by an outward proton gradient. The transcript level of NHE-1 was measured by reverse transcription-polymerase chain reaction in comparison with a constitutively expressed reference gene (beta-actin). Intracellular pH was lower in hypertensive patients than normotensive subjects (7.34+/-0.01 versus 7.39+/-0.01, mean+/-SEM, P
