Silencing of the gene coding for the ϵ subunit of DNA polymerase III slows down the growth rate of Escherichia coli populations
Silencing of the gene coding for the ϵ subunit of DNA polymerase III slows down the growth rate of Escherichia coli populations
复制标题
DNA 聚合酶 III ε 亚基编码基因的沉默会减慢大肠杆菌群体的生长速度
DOI：
10.1016/s0014-5793(03)00604-5
复制
发表时间：
2003
期刊：
影响因子：
3.5
通讯作者：
A. Hochkoeppler
中科院分区：
文献类型：
--
作者：
Alessandra Stefan;L. Reggiani;S. Cianchetta;A. Radeghieri;A. Gonzàlez Vara y Rodriguez;A. Hochkoeppler
大肠杆菌中的染色体复制是由多聚体酶DNA聚合酶III完成的;已经评估了DNA聚合酶III的持续合成能力和保真度的α亚基（由dnaQ编码）在体内的相关性。为此目的，通过体内表达不同的反义RNA使dnaQ有条件地沉默。出乎意料的是，Shine-Dalgarno序列的存在对于反义构建体的有效性是必需的。沉默的dnaQ诱导生长速率的严重下降，而不是由高突变频率，这表明α亚基主要影响DNA聚合酶III的持续合成能力。
Chromosome replication in Escherichia coli is accomplished by the multimeric enzyme DNA polymerase III; the relevance, in vivo, of the ϵ subunit (encoded by dnaQ) for processivity and fidelity of DNA polymerase III has been evaluated. To this aim, dnaQ has been conditionally silenced by means of in vivo expression of different antisense RNAs. Unexpectedly, the presence of the Shine–Dalgarno sequence is essential for the effectiveness of antisense constructs. Silencing of dnaQ induces a severe decrease in growth rate not paralleled by high mutation frequencies, suggesting that the ϵ subunit primarily affects the processivity of DNA polymerase III.