Comparison of different rearranged immunoglobulin kappa genes of a myeloma by electronmicroscopy and restriction mapping of cloned DNA: implications for "allelic exclusion".
Comparison of different rearranged immunoglobulin kappa genes of a myeloma by electronmicroscopy and restriction mapping of cloned DNA: implications for "allelic exclusion".
复制标题
通过电子显微镜和克隆 DNA 限制性图谱比较骨髓瘤的不同重排免疫球蛋白 kappa 基因：对“等位基因排除”的影响。
DOI：
10.1093/nar/8.20.4689
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发表时间：
1980
影响因子：
14.9
通讯作者：
Zentgraf,H
中科院分区：
文献类型：
--
作者：
Walfield,AM;Storb,U;Selsing,E;Zentgraf,H
We have studied the organization and function of different. rearranged kappa genes in a myeloma, MOPC-21. Two kappa genes were cloned into Charon 4A and compared with each other and with a cloned germline CKgene by restriction mapping and electron microscopy. One MOPC-21 clone corresponds to the gene coding for the MOPC-21 kappa chain polypeptide; it has the V21gene joined with the CKgene at the J2 sequence. The other MOPC-21 clone corresponds to a nonfunctional rearranged MOPC-21 kappa gene, except for a 1kb deletion, 3' of J4. A similar deletion is also found in a “new” kappa gene present in MS-1, a cellular subclone of MOPC-21. The clone of the “nonfunctional” kappa gene has a V gene which is distinct from V21which is joined to CKin the vicinity of J2. The undeleted form of this gene codes for a kRNA having the size of mature kmRNA which, however, is not translated into kappa chains. Thus the defect of the “nonfunctional” gene manifests itself at a late step of gene expression. The basis for “allelic exclusion” of antibody genes may simply be the complexity of the processes between genes and gene products, resulting in the expression of only one gene.
