Random amplified polymorphic DNA (RAPD) analysis reveals genetic relationships among the annual Cicer species
Random amplified polymorphic DNA (RAPD) analysis reveals genetic relationships among the annual Cicer species
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DOI：
10.1007/s001220051117
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发表时间：
1999-03-01
影响因子：
5.4
通讯作者：
Ahmad, F
中科院分区：
文献类型：
--
作者：
Ahmad, F
用随机扩增多态DNA标记区分了代表栽培鹰嘴豆的9个不同的Cester分类群和其他8个相关的一年生野生种。在测试的75个随机10聚体引物中，只有8个在所有物种中扩增了基因组DNA。共扩增出115个重复性好的标记，除1个为多态标记外，其余均为多态标记，用于推断一年生西瓜种间的亲缘关系。共观察到4个不同的聚类群，第一类群分别代表华枝锦鸡儿、网锦锦鸡儿和棘球锦鸡儿，其次是华山锦鸡儿和山毛锦鸡儿，第三类群是羽冠锦鸡儿、朱砂锦鸡儿和华山锦鸡儿。楔形冰草没有与任何物种聚在一起，与栽培物种的位置最远。所推论的物种间的亲缘关系与前人的研究基本一致。此外，还鉴定了针对所有9个物种的物种诊断扩增产物。这些结果清楚地证明了一种基于随机引物DNA扩增的方法学，可用于研究雪梨的系统发育和鹰嘴豆的改良。
Random amplified polymorphic DNA markers were used to distinguish between nine different Cicer taxa representing the cultivated chickpea and eight other related annual wild species. Of the 75 random10-mer primers tested, only 8 amplified genomic DNA across all the species. A total of 115 reproducibly scorable RAPD markers were generated, all except 1 polymorphic, and these were utilized to deduce genetic relationships among the annual Cicer species. Four distinct clusters were observed and represented C. arietinum, C. reticulatum and C. echinospermum in first cluster followed by C. chorassanicum and C. yamashitae in the second cluster, while C. pinnatifidum, C. judaicum and C. bijugum formed the third cluster. Cicer cuneatum did not cluster with any of the species and was most distantly placed from the cultivated species. Except for the placement of C. chorassanicum and C. yamashitae, deduced species' relationships agreed with previous studies. In addition, species-diagnostic amplification products specific to all the nine species were identified. The results clearly demonstrate a methodology based on random-primed DNA amplification that can be used for studying Cicer phylogeny and chickpea improvement.