In vivo confocal Raman microspectroscopy of the skin:: Noninvasive determination of molecular concentration profiles
In vivo confocal Raman microspectroscopy of the skin:: Noninvasive determination of molecular concentration profiles
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DOI：
10.1046/j.1523-1747.2001.01258.x
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发表时间：
2001-03-01
影响因子：
6.5
通讯作者：
Puppels, GJ
中科院分区：
文献类型：
--
作者：
Caspers, PJ;Lucassen, GW;Puppels, GJ
共焦拉曼光谱作为一种非侵入性的体内光学方法被引入到皮肤中的分子浓度分布测量中。它示出了它如何可以被应用于确定在角质层中的水浓度作为到皮肤表面的距离的函数，具有5 μ m的深度分辨率。所得到的体内浓度曲线与已发表的数据在定性和定量上一致，这些数据是通过对皮肤样品进行体外X射线微量分析获得的。半定量的浓度分布测定的主要成分的天然保湿因子（丝氨酸，甘氨酸，吡咯烷酮-5-羧酸，精氨酸，鸟氨酸，瓜氨酸，丙氨酸，组氨酸，尿刊酸）和汗液成分乳酸和尿素。详细描述的信号分析方法，使皮肤拉曼光谱的这种信息的提取。没有其他非侵入性的体内方法存在，使皮肤分子组成的分析作为到皮肤表面的距离的函数，具有类似的细节和空间分辨率。因此，可以预期的是，在体内共焦拉曼光谱将发现许多应用在基础和应用皮肤病学研究。
Confocal Raman spectroscopy is introduced as a noninvasive in vivo optical method to measure molecular concentration profiles in the skin. It is shown how it can be applied to determine the water concentration in the stratum corneum as a function of distance to the skin surface, with a depth resolution of 5 mum. The resulting in vivo concentration profiles are in qualitative and quantitative agreement with published data, obtained by in vitro X-ray microanalysis of skin samples. Semi-quantitative concentration profiles were determined for the major constituents of natural moisturizing factor (serine, glycine, pyrrolidone-5-carboxylic acid, arginine, ornithine, citrulline, alanine, histidine, urocanic acid) and for the sweat constituents lactate and urea. A detailed description is given of the signal analysis methodology that enables the extraction of this information from the skin Raman spectra. No other noninvasive in vivo method exists that enables an analysis of skin molecular composition as a function of distance to the skin surface with similar detail and spatial resolution. Therefore, it may be expected that in vivo confocal Raman spectroscopy will find many applications in basic and applied dermatologic research.