Nanosized zinc oxide particles induce neural stem cell apoptosis
Nanosized zinc oxide particles induce neural stem cell apoptosis
复制标题
纳米氧化锌颗粒诱导神经干细胞凋亡
DOI：
10.1088/0957-4484/20/11/115101
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发表时间：
2009-03-18
期刊：
影响因子：
3.5
通讯作者：
Jiao, Zheng
中科院分区：
文献类型：
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作者：
Deng, Xiaoyong;Luan, Qixia;Jiao, Zheng
鉴于纳米级氧化锌 (ZnO) 材料在我们生活中的广泛应用，人们越来越担心其对健康和环境的无意影响。在这项研究中，研究了不同尺寸的氧化锌纳米粒子对小鼠神经干细胞（NSC）的神经毒性。细胞活力测定表明，ZnO 纳米粒子对 NSCs 表现出剂量依赖性的毒性作用，但没有尺寸依赖性的毒性作用。通过共聚焦显微镜、透射电镜检查和流式细胞术观察和分析凋亡细胞。所有结果都支持ZnO纳米颗粒毒性来自于培养基或细胞内溶解的Zn2+的观点。我们的结果强调在使用和处置 ZnO 制造的纳米材料期间需要谨慎，以防止意外的环境和健康影响。
Given the intensive application of nanoscale zinc oxide (ZnO) materials in our life, growing concerns have arisen about its unintentional health and environmental impacts. In this study, the neurotoxicity of different sized ZnO nanoparticles in mouse neural stem cells (NSCs) was investigated. A cell viability assay indicated that ZnO nanoparticles manifested dose-dependent, but no size-dependent toxic effects on NSCs. Apoptotic cells were observed and analyzed by confocal microscopy, transmission electron microscopy examination, and flow cytometry. All the results support the viewpoint that the ZnO nanoparticle toxicity comes from the dissolved Zn2+ in the culture medium or inside cells. Our results highlight the need for caution during the use and disposal of ZnO manufactured nanomaterials to prevent the unintended environmental and health impacts.