The radish Rfo restorer gene of Ogura cytoplasmic male sterility encodes a protein with multiple pentatricopeptide repeats
The radish Rfo restorer gene of Ogura cytoplasmic male sterility encodes a protein with multiple pentatricopeptide repeats
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DOI：
10.1046/j.1365-313x.2003.01799.x
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发表时间：
2003-07-01
期刊：
影响因子：
7.2
通讯作者：
Landry, BS
中科院分区：
文献类型：
--
作者：
Brown, GG;Formanová, N;Landry, BS
利用萝卜核基因RFO恢复甘蓝型油菜Ogura细胞质雄性不育(CMS)。利用萝卜与拟南芥之间的同步性，采用基于图谱的克隆方法克隆了RFO。一个编码687氨基酸蛋白的萝卜基因被发现在转化甘蓝型油菜后具有雄性可育性，该基因具有预测的线粒体靶向前序列。这个基因，就像最近描述的矮牵牛RF基因一样，编码一个含有五肽重复(PPR)的蛋白，在这种情况下有多个PPR结构域，在这种情况下是16个。在该基因的两侧有两个类似的基因，它们似乎不起RFO的作用。将该RFO区与拟南芥的Syntenic区进行比较，结果表明在拟南芥的RFO等效位点处不存在PPR基因，但在该位置发现了一个相对较小的PPR基因，约40kb。讨论了这些发现对恢复基因和其他PPR编码基因进化的影响。
A single radish nuclear gene, Rfo, restores Ogura (ogu) cytoplasmic male sterility (CMS) in Brassica napus. A map-based cloning approach relying on synteny between radish and Arabidopsis was used to clone Rfo. A radish gene encoding a 687-amino-acid protein with a predicted mitochondrial targeting pre-sequence was found to confer male fertility upon transformation into ogu CMS B. napus. This gene, like the recently described Petunia Rf gene, codes for a pentatricopeptide repeat (PPR)-containing protein with multiple, in this case 16, PPR domains. Two similar genes that do not appear to function as Rfo flank this gene. Comparison of the Rfo region with the syntenic Arabidopsis region indicates that a PPR gene is not present at the Rfo-equivalent site in Arabidopsis, although a smaller and related PPR gene is found about 40 kb from this site. The implications of these findings for the evolution of restorer genes and other PPR encoding genes are discussed.