Controlling the cellular uptake of gold nanorods
Controlling the cellular uptake of gold nanorods
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DOI：
10.1021/la062642r
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发表时间：
2007-02-13
期刊：
影响因子：
3.9
通讯作者：
Wei, Alexander
中科院分区：
文献类型：
--
作者：
Huff, Terry B.;Hansen, Matthew N.;Wei, Alexander
用十六烷基三甲基溴化铵（CTAB），一种用于纳米棒合成的阳离子胶束表面活性剂涂覆的金纳米棒，通过非特异性摄取机制被KB细胞快速且不可逆地内化。通过双光子发光（TPL）显微镜监测细胞表面附近的内化纳米棒，并观察到以二次扩散速率向细胞核迁移。内化的纳米棒没有被排出，而是在细胞内形成永久性聚集体，这些聚集体保持健康并在5天内生长至汇合。通过用化学吸附表面活性剂取代CTAB表面活性剂层，特别是通过使用原位二硫代氨基甲酸盐形成将聚（乙二醇）链缀合到纳米棒上，可以大大减少非特异性纳米棒摄取。
Gold nanorods coated with cetyltrimethylammonium bromide (CTAB), a cationic micellar surfactant used in nanorod synthesis, were rapidly and irreversibly internalized by KB cells via a nonspecific uptake mechanism. Internalized nanorods near the cell surface were monitored by two-photon luminescence (TPL) microscopy and observed to migrate toward the nucleus with a quadratic rate of diffusion. The internalized nanorods were not excreted but formed permanent aggregates within the cells, which remained healthy and grew to confluence over a 5-day period. Nonspecific nanorod uptake could be greatly reduced by displacing the CTAB surfactant layer with chemisorptive surfactants, particularly by the conjugation of poly(ethylene glycol) chains onto nanorods using in situ dithiocarbamate formation.