Multi-functional crosslinked Au nanoaggregates for the amplified optical DNA detection
Multi-functional crosslinked Au nanoaggregates for the amplified optical DNA detection
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用于放大光学 DNA 检测的多功能交联金纳米聚集体
DOI：
10.1016/j.bios.2009.04.029
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发表时间：
2009-07-15
影响因子：
12.6
通讯作者：
Fan, Chunhai
中科院分区：
文献类型：
--
作者：
Li, Jiang;Song, Shiping;Fan, Chunhai
我们报告了一种新的三明治型检测DNA的光学检测使用多组分交联Au纳米颗粒聚集体。在该设计中，DNA桥接的多功能Au纳米颗粒聚集体，它集成了DNA识别（检测探针），信号放大（酶，辣根过氧化物酶）和非特异性阻断（牛血清白蛋白，BSA）部分，被用作检测探针。夹心式检测包括固定在磁性微粒上的捕获探针、靶和Au纳米颗粒聚集体检测探针。在典型的传感过程中。通过DNA杂交将Au聚集体检测探针带到磁性颗粒附近。一旦加入磁场，这些夹心复合物就会被磁性分离。结果表明，限制在Au聚集体表面的HRP可以催化酶底物并产生光信号。该检测方法用于检测乳腺癌相关BRCA-1基因。检测限约为1 fmol，与单个Au纳米颗粒标记法相比，检测限有了显著提高。因此，我们的研究结果解决了Au纳米聚集体作为DNA检测平台的可能性。(C)2009爱思唯尔有限公司版权所有。
We report a novel sandwich type assay for the optical detection of DNA using multi-component crosslinked Au nanoparticle aggregates. In this design, a DNA bridged multi-functional Au nanoparticle aggregate, which integrates DNA recognition (detection probe), signal amplification (enzyme, horseradish peroxidase) and non-specific blocking (bovine serum albumin, BSA) section, is employed as detection probe. The sandwich type assay includes the capture probe that immobilized on magnetic microparticles, target and the Au nanoparticle aggregates detection probe. In a typical sensing process. the Au aggregates detection probe is brought to the proximity of magnetic particles through the DNA hybridization. Once magnetic field is added, these sandwich complexes are magnetically separated. As a result, HRP that confined at the surface of Au aggregates could catalyze the enzyme substrate and generate an optical signal. This assay is employed for the detection of breast cancer-associated BRCA-1 gene. The detection limit is about 1 fmol, which is significantly improved as compared with the results obtained from individual Au nanoparticle labeled assay. Thus, our results address the possibility of Au nanoaggregates as platform for DNA detection. (C) 2009 Elsevier B.V. All rights reserved.