Structural biology of TRP channels.
Structural biology of TRP channels.
复制标题
DOI：
10.1007/978-3-319-05161-1_10
复制
发表时间：
2014
影响因子：
--
通讯作者：
Gaudet, Rachelle
中科院分区：
文献类型：
--
作者：
Hellmich, Ute A;Gaudet, Rachelle
膜蛋白仍然是结构生物学家具有挑战性的目标，尽管最近的技术发展有关样品制备和结构测定。我们回顾了最近的进展，对TRP通道的结构理解和用于此目的的技术。我们讨论了电子显微镜（EM），X-射线晶体学和核磁共振（NMR）的可用低分辨率结构，并回顾了由此产生的见解TRP通道功能的各种亚家族成员。TRPV 1最近的高分辨率结构将在第十章中详细讨论。我们还考虑了利用积累的TRP和同源蛋白的结构信息推导不同TRP通道亚家族的全长结构的机会和挑战，如建立同源模型。最后，我们总结了“圣杯”的原子细节的理解TRP通道的不同功能的前景。
Membrane proteins remain challenging targets for structural biologists, despite recent technical developments regarding sample preparation and structure determination. We review recent progress towards a structural understanding of TRP channels and the techniques used to that end. We discuss available low-resolution structures from electron microscopy (EM), X-ray crystallography and nuclear magnetic resonance (NMR), and review the resulting insights into TRP channel function for various subfamily members. The recent high-resolution structure of TRPV1 is discussed in more detail in Chapter X. We also consider the opportunities and challenges of using the accumulating structural information on TRPs and homologous proteins for deducing full-length structures of different TRP channel subfamilies, such as building homology models. Finally, we close by summarizing the outlook of the “holy grail” of understanding in atomic detail the diverse functions of TRP channels.