Biohardening with Plant Growth Promoting Rhizosphere and Endophytic bacteria induces systemic resistance against Banana bunchy top virus
Biohardening with Plant Growth Promoting Rhizosphere and Endophytic bacteria induces systemic resistance against Banana bunchy top virus
复制标题
DOI：
10.1016/j.apsoil.2007.12.006
复制
发表时间：
2008-06-01
影响因子：
4.8
通讯作者：
Samiyappan, R.
中科院分区：
文献类型：
--
作者：
Harish, S.;Kavino, M.;Samiyappan, R.
香蕉组培已成为全球领先的农业基础产业技术之一。然而，由于香蕉植物的柔韧性和在自然环境中缺乏抵抗力，它们更容易受到病虫害的影响。香蕉束顶病（BBTD）是由香蕉束顶病毒（BBTV）引起的香蕉和大蕉最严重的病毒性病害。生物熏蒸（或）生物硬化是指用微生物接种剂处理香蕉组织培养植株，以增强植株对生物和非生物胁迫的抵抗力。从香蕉的球茎和根中分离到40株植物生长促进内生细菌（PGPE），并对其诱导抗BBTV的能力进行了试验。内生细菌的表型和分子特征显示存在假单胞菌和芽孢杆菌。采用ELISA、DIBA和PCR检测香蕉小苗是否存在BBTV，并用两种根瘤菌（荧光假单胞菌、Pf1、CHA0）和内生细菌（EPB5、EPB22）对未感染的香蕉植株进行生物硬化。在田间条件下，用根瘤菌和内生菌混合配方（EPB5 + EPB22 + Pf1 + CHAO）处理的植株可显著降低BBTV，侵染率为33.33%，比对照降低60%。根际和内生菌制剂处理植株防御相关酶和致病相关蛋白的表达量高于对照植株。处理后植株的形态和生理特征也很明显，表明其具有促进植株生长的作用。因此，本研究清楚地表明，PGPR和PGPE介导的香蕉植物对BBTV的系统性抗性在BBTD的管理中具有潜在的作用。(c) 2008 Elsevier B.V.版权所有
Tissue culture banana has emerged as one of the leading global agro based industrial technologies. However, the banana plants are found to be more susceptible to pests and diseases due to their pliable nature and lack of withstanding in the natural environment. Banana bunchy top disease (BBTD) caused by Banana bunchy top virus (BBTV) is the most serious virus disease of banana and plantain world wide. Biopriming (or) biohardening refers to treatment of tissue culture banana plantlets with microbial inoculants to strengthen the plantlets against biotic and abiotic stresses. Forty plant growth promoting endophytic bacteria (PGPE) were isolated from the corm and roots of banana and tested for the ability to induce systemic resistance against BBTV. Phenotypic and molecular characterization of the endophytic bacteria revealed the presence of Pseudomonas and Bacillus species. Micropropagated banana plantlets were tested for the presence of BBTV by ELISA, DIBA and PCR and the uninfected plants were biohardened with two rhizobacterial (Pseudomonas fluorescens, Pf1, CHA0) and endophytic bacterial (EPB5, EPB22) strains. Plants treated with mixtures of rhizobacterial and endophytic bacterial formulations viz., EPB5 + EPB22 + Pf1 + CHAO was significantly effective in reducing BBTV under field conditions recording 33.33% infection with 60% reduction over control. The expression of defense-related enzymes and pathogenesis related proteins were more in the plants treated with rhizosphere and endophytic bacterial formulations than the control plants. The morphological and physiological characters were also well pronounced in the treated plants indicating its plant growth promoting nature. Thus, the present study clearly demonstrated that PGPR and PGPE mediated induction of systemic resistance in banana plants against BBTV have the potential role in the management of BBTD. (c) 2008 Elsevier B.V. All rights reserved.